Radiotherapy Sensitization Role Of Curcumin On Hela Cell Line Of Cervical Cancer And Its Possible Mechanism

Objective：To investigate the radiotherapy sensitization roleof Curcumin on Hela cell line of cervical cancer and itspossible mechanism.For curcumin can applied to clinical ascervical cancer radiotherapy sensitization agent providetheoretical basis.Methods:(1)The human cervical squamous carcinoma Hela cellline was used. Tetrazolium salt Colorimetric Experiment (MTT)was used to detection curcumin,radioactive rays singleacting,and combined them application on the influence ofcervical cancer Hela cell proliferation.Determine theradiation best work dose and curcumin best workconcentration.(2)Clone forming assay was carried out toobserve the radiotherapy sensitization role of Curcumin on Helacell line of cervical cancer, and colonies with cells exceeded50were calculated on inverted microscope. take count of cellsurvival fraction(SF). Colony forming efficiency（CFE）was thenumber of colonies with above50cells/the number inoculated.The cell survival rate=(CFEexperimental groups/CFEcontrol group)×100%.Comparative tumorigenic ability after radioactive rays single acting with joint action of curcumin.(3)Cell cycle andthe rate of apoptosis of the human cervical squamous carcinomaHela cell line was determined by flow cytometry,which arethrough curcumin,X-rays single acting,and combined themapplication.And the results were analyzed by the multicyclesoftware.(4)Western-blot technique detected the expression ofP53、P21、Bax and Bcl-2protein after the cervical cancer Helacells were treated.Results：(1)Curcumin could inhibit the proliferation of Helacells in a time and dose dependent manner. when Curcumin andradiation were used together on Hela at12Gy dose radiation and3.125-25μmol／L Curcumin on Hela cell line, the cytotoxiceffect was obviously in a dose and time dependent manner within48hours;However,Curcumin showed no more cytotoxic effect whenconcentration was over25μmol／L.(2)Different doses ofradiation alone role,and it conbine with curcumin, all caninhibit colony formation of human cervical cancer Helacells,and joint application is more significant than rayinhibit alone.Thus curcumin is able to enhance radiation effecton human cervical carcinoma Hela cells.(3)Compare with controlgroup,curcumin and radioactive can make cell cycle arrest inradiation sensitivity in G2/M phase and induce apoptosis.Thiseffect was much more obvious when irradiation and curcumin werecombined than either of them was used alone.(4)The protein ofP53, P21were increased and the ratio of Bcl-2/Bax was decreasedwhen curcumin and irradiation were applied to cellsalone(p<0.05), and this change were much more obvious whenirradiation and curcumin were combined than either of them wasused alone（p<0.01）. Conclusion：(1)Curcumin and X-ray has cytotoxic effect tocervical cancer Hela cells.(2)Curcumin have radiotherapysensitization effect on cervical cancer Hela cells.(3)Themechanism of curcumin enhance radiosensitivity on Hela cellsmay be due to its regulation on cell cycle,induce apoptosis andup-regulating the expression of P53,P21,Bax Protein anddown-regulating the expression of Bcl-2protein.