Study On The Expression Of FLT3 And N-ras Gene MRNA In Human Leukemic Stem Cell By Using The Tranditional Medicine Fomular

Objectives: To observe the expression of FLT3 and N-ras mRNA in Leukemic Stem Cell(LSC) by using the tranditional medicine fomular,and discuss the regulation mechanism of tranditional medicine fomular targeted intervention in LSC.Methods: We collected the bone marrow of the 50cases of AML,peripheral leukemic stem cell from human leukemic bone marrow,and culture the cell,the cell suspension was divided into four groups(the blank group without the drug,the experimental group,respectively,add the Yuanfang fomular,Fuzheng fomular,Quxie fomular).Through reverse transcription- polymerase chain reaction (RT-PCR), we observe the expression level of FLT3 and N-ras gene mRNA in LSC by using the tranditional medicine fomular. Results: (1)The expression of FLT3 and N-ras in Yuanfang fomular group were lower obviously than blank control group,Fuzheng fomular group and the Quxie fomular group(P<0.01);Yuanfang fomular can significantly reduce the expression of FLT3 and N-ras gene;There was no significant difference between the expression of Fuzheng fomular group and the Quxie fomular group(P>0.05), the strongest expression level is blank control group, the lowest expression level is Yuanfang fomular group, and the expression level of Fuzheng fomular group and the Quxie fomular group between the two groups.(2) There was no significant difference among the FAB subtypes (P>0.05).(3) The positive expression FLT3 and N-ras was associated with a higher white cell count(P<0.05),a higher percentage of bone marrow blast cells(P<0.05).Conclusion: The expression of FLT3 and N-ras mRNA in human leukemias is discreased by using the Yuanfang fomular. The possible mechanism is that lower FLT3 and N-ras gene expression, and provide a reliable basis for tranditional medicine fomular targeted intervention in LSC.