Effects Of Aprotinin On Expression Of IL-8 In Human Umbilical Vein Endothelial Cells Stimulated By Thrombin

Objective To investigate whether aprotinin could inhibit IL-8 expression of human umbilical vein endothelial cells and the NF-κB translocation from cytoplasm to nucleus which stimulated by thrombin.Methods Cultured cells were assigned to four experimental groups, including control group, thrombin only group, 800KIU/ml aprotinin(half dosage)plus thrombin group, and 1600KIU/ml aprotinin (full dosage) plus thrombin group. The levers of soluble IL-8 in supernatant of cultured cells were measured by Elisa, real-time fluorescence quantitative PCR was applied to assay the expression of IL-8 mRNA levers, and the distribution of NF-κB in cytoplasm and nucleus was detected by laser confocal microscopy.Results⑴Thrombin can stimulate HUEVCs excreting IL-8, as well as the activation and nucleus translocation of NF-κB(P<0.01, P<0.01).⑵Both half dosage and full dosage aprotinin can inhibit the production of IL-8 and the translocation of NF-κB (P<0.01,P<0.01), no significant difference was observed between these two groups(P>0.05).Conclusions Thrombin can upregulate the HUVECs'IL-8 expression and activate NF-κB, both half and full dosage aprotinin can inhibit this action of thrombin, as well as the translocation of NF-κB from cytoplasm to nucleus triggered by thrombin.