Expression And Significance Of CD105 And SSTR5 In Laryngeal Carcinoma

Objective: Laryngeal carcinoma is the most common malignant tumor in throat, its incidence has a significant growth trend. Malignant tumors at the occurrence, development and transfer process, One of the key factors is must form a functional vascular system to supply oxygen necessary for its growth and nutritional substances, Therefore, angiogenesis is a malignant foundation for tumor growth, invasion and metastasis . CD105 is better pan-vascular marker of a vascular endothelial marker than CD31, CD34.CD105-IMVD of laryngeal Microvascular Medium is correlated significantly with the proliferation of laryngeal cancer, stage and metastasis, Somatostatin (SS) is primarily through somatostatin receptor (SSTR) role, it has inhibited tumor cell growth and induce apoptosis and inhibit tumor angiogenesis. This experiment detectes the expression of SSTR5 in laryngeal squamous cell carcinoma and adjacent tissues by immunohistochemistry method in order to evaluat the expression of SSTR5 differences in laryngeal carcinoma and adjacent tissues, and to explore the relations of tumor occurrence and development for a view to somatostatin and its analogues used in the treatment of laryngeal cancer based on the provision. The expression of CD105 and SSTR5 is inversely proportional in laryngeal carcinoma, detection of CD105 and SSTR5 is a good guide to application for anti-angiogenesis agents and SST and SSTA laryngeal. Method:1 It detectes the expression of CD105 and SSTR5 in40 cases of laryngeal carcinoma and 40 cases of adjacent tissues by application of immunohistochemical methods, and to explore the expression of CD105 and SSTR5 relations.2 We made t test, non-parametric rank sum test and analysis of factors related to both for the data by application of the data SPSS (11.5) statistical software package, P <0.05 has statistical significance.Results:All materials are derived from the provincial hospital in Hebei Province alone after surgical treatment of laryngeal cancer tissue samples of patients (all the patients had not received preoperative radiotherapy, chemotherapy and immunotherapy), all specimens were drawn within 24 hours, 10% neutral formalin-fixed. All specimens were pathologically confirmed to be squamous cell carcinoma. Pathology by two physicians will be graded in all cases, Well-differentiated squamous cell carcinoma exists many Keratocyst beads, intercellular bridge; Poorly differentiated squamous cell carcinoma often lacks of or only has a small number of cells exist diagonalization, intercellular bridge; in the differentiation of squamous cell carcinoma in between, Well-differentiated squamous cell carcinoma has 19 cases, 14 cases of differentiated cases, 7 cases of poorly differentiated squamous cell carcinoma; There is 11 cases of lymph node metastasis , 29 cases of no lymph node metastasis ; 36 cases of male patients, four cases of female patients; Patients younger than 50-year-old of 15 cases, age greater than 50-year-old of 25 cases; In accordance with international anti-cancer association (UICC) 2002 announced a phased program of the TNM classification, T1 10 Example: T2 period 15 cases: T3 period of nine cases; T4 period of six cases. Another check for at least five millimeter away from the cancer tissue of the adjacent tissues as controls(all non-cancer cells confirmed by pathology).1 The expression of CD105 in laryngeal carcinomaCD105 organizations mainly expressed in the tumor micro-vascular endothelial cells, cancer organization will be a strong brown dye microvascular, it is mainly concentrated at the edge of the tumor tissue staining, while the large vascular staining was not obvious. In the control group, no expression. Laryngeal carcinoma CD105-IMVD was 6.63±1.79, corresponding adjacent tissues of the IMVD for 0, comparing the two groups, the difference was significant (p<0.01). In laryngeal carcinoma the value of CD105-IMVD of patients aged less than 50 is 6.36±1.99, at the age of the patients with more than 50 of the value of CD105-IMVD is 6.79±1.69, comparing the two groups p>0.05, the difference was not statistically significant; Male, female patients with CD105-IMVD the values were6.61±1.83,6.85±1.67, comparing the two groups p>0.05, the difference was not statistically significant; The values of CD105-IMVD in well-differentiated groups, and poorly differentiated laryngeal group were 6.51±2.05,6.74±1.57,comparing the two groups p>0.05, the difference was not statistically significant; The value of CD105-IMVD in T1T2 period and T3T4 period laryngeal group were 5.76±1.63,8,08±0.90; CD105-IMVD in period T3T4 laryngeal squamous cell carcinoma was significantly higher than T1T2 period, the difference was significant (p<0.01); There is CD105-IMVD is 8.53±1.38 in lymph node metastasis of laryngeal carcinoma, but 5.91±1.36 in no lymph node metastasis of laryngeal carcinoma; The values of CD105-IMVD in lymph node metastasis group higher than those without lymph node metastasis group,2 The expression of SSTR5 in laryngeal carcinomaLaryngeal SSTR5 protein expression rate was 77.5%, cancer adjacent tissues SSTR5 protein expression rate was 90.0%, The positive expression rate of the difference was not significant(p>0.05), but obvious differences in expression intensity(p>0.05).The relations of SSTR5 and clinical pathological factors.SSTR5 expression intensity has nothing to do in age, sex and Lymph node metastasis or not, however, in different clinical stages, pathological degree of tumor differentiation Organize SSTR5 expression were significantly different. Well-differentiated squamous cell carcinoma of SSTR5 expression intensity is higher than in poorly differentiated(p>0.05).T1T2 squamous cell carcinoma phase SSTR5 expression intensity was significantly higher than that T3T4 period(p<0.01).Conclusion:1 CD105 and tumor angiogenesis is closely related to activity, CD105 is a sign of tumor angiogenesis, CD105 antibodies can be used as an ideal tumor microvascular endothelial cell antigen-specific marker antibodies, is expected to replace the pan-endothelial cell markers for the detection of IMVD, tumor angiogenesis research to have a good broad application prospects. CD105-IMVD of laryngeal carcinoma value will be a prediction of recurrence and metastasis of laryngeal cancer in patients with essential indicators. At present, the inhibition of tumor angiogenesis targeting therapy has become a hot research, and CD105 is an ideal target for inhibiting angiogenesis.2 This study researchs the expression of SSTR5 in laryngeal cancer tissue and the adjacent tissues, to explain the expression of SSTR5 in the majority of laryngeal tumor cells .Application of SST and SSTA probably can effectively inhibit tumor cell proliferation. SSTR5 expression in response to moderate intensity or strong positive laryngeal, SST and SSTA are possible effective treatment. SSTR5 expression and the lack of or only weakly positive expression of the larynx are probably less effective treatment. Identification of SSTR5 in laryngeal carcinoma Whether positive expression can be provided for the clinical significance of the following aspects:(1) Whether the application can guide the treatment of laryngeal cancer Somatostatin and its analogues; (2) It can determine whether the application of somatostatin scintigraphy technology (SRS) to distinguish laryngeal SSTR-positive lesions and metastases; (3) It can be applied to carry radioactive material and the somatostatin analogue, on SSTR-positive laryngeal line targeted radiotherapy.3 CD105 and SSTR5 joint application The Relevance of CD105 and SSTR5 in laryngeal cancer by statistical methods, r=0.345, That expression of CD105 is egatively related with SSTR5 in laryngeal cancer.CD105 is a more than CD31,CD34 better microvascular endothelial cell markers with specificity; Cancer cells have the majority of laryngeal SSTR5 expression,application of SST and SSTA probably can effectively inhibit tumor cell proliferation. CD105-IMVD values higher, and the expression of SSTR5 weaker intensity, its effect of the application of SST and SSTA is better to anti-tumor growth and anti-angiogenic . Detection of CD105 and SSTR5 expressions in laryngeal application has a good guide for anti-angiogenesis agents and SST and SSTA.