Correlation Between Interleukin-1, 4 Gene Polymorphism And Cerebral Infarction

Object: Cerebral infarction (CI) is a common and frequently encountered disease of people in our country,which causes high multilation and fatality rate.In recent years there are more and more young people suffering the cerbral infarction disease.Many risk factors cause CI,for example high blood pressure,hyperlipemia,diabetes,cardiac disease,smoking,alcohol abuse and so on.By controling those mentioned risk factors of cerbral infarction can obviously delay and cut down the development of CI.However in clinical practice,we find that so many people who have above mentioned risk factors don't suffer from CI, But some people who don't have those mentioned risk factors suffered from cerebral infarction disease .So this phenomenon illustrates that there may be some hereditary factors associating with CI.An external study shows that the people whose parents suffered from CI disease is liability to have CI than those people whose parents don't have.So lots of scholars generally consider that CI is a disease of inheritance disease lot's of factors plays an important part in which .These years predisposing genes associate with CI and their polymorphisms become to be the multiple and hot point in the sphere of heredity mechanism. A great quantity of animal experiments and experiments whice take place in and out of body discover that inflammafory reaction plays an important role in the process of artherosclerosis,cerebral infarction,ischemia-reperfusion injury and so on.These factors which participate in the inflammatory reaction include tumor nercrosis factor (TNF),interferon(IFN),interleukin-1(IL-1),interleu -kin-4(IL-4),interleukin-10(IL-10),transforming growth factor (TGF) and so on.IL-1,TNF are thought to be the primary inflammatory cells,and IL-4,IL-10 are anti-inflammatory cells. TNF,IL-1,IL-4,IL-10 all have gene polymorphiems. The purposes of this experiment is to search much more evidences for cerebral infarction's genetic risk factors.At the same time we can explor the way of using gene diagnosis in cerebral infarction.Methods: 1 The cerebral infarction group(CI group) is composed of 41 patients,we record whether they have high blood pressure,diabetes,hyperlipemia,smoking and alcohol abuse.At the same time 41 people who taked healthy examinations in our hospital were selected as the healthy control group(control group).2 The peripheral venous blood of all the selected objects are draw-off in the morning when they don't have breakfast,the blood are anticoagulated by EDTA.And then DNA genes of the objects were isolated with kit.3 The interesting fragments were amplified by polymerase chain reaction.All PCR products were observed by agarose gel electrophoresis and then are analyzed.4 The PCR products were digested by restriction enzymes and analyzed after eletroph -oresis. Five mutations respectively at IL-1α-889 from C to T, IL-1β+3953 from C to T,IL-1β-511 from C to T,IL-4-590 from C to T and IL-4+33 from C to T were determined in objects screened by a combination of polymerase chain reaction(PCR) amplification and restriction fragament length polymorph -ism(RFLP).5 Some samples selected at random were sequenced to prove the products digested by incision enzymes.6 Correlation analysis:first Hardy-Weinberg testing method is used to confirm that the two groups can be presentative of the overall population.Then we use the statistical analysis software to analyse. The number of every genetype and allelomorphic gene are straightly counted,then accounting the gene frequency.The x2 test is used to compare the datas between the tow groups.The aim is to analyse the relationship of gene polymorphism and the cerebral infarction. Logistic regression was adopted to adjusted some common and significant stroke risk factors,the aim is to observe that whether the gene polymorphism is the independent risk factor of the cerebral infarction.Results: 1 Every groups'gengtype distributions are demon -strated that they can presentate all population by using Hardy-Weinberg testing method(P>0.05). 2 The CC,CT,TT genetype distributions of IL-lα(-889C/T) in the CI group are 56.10%,31.71%,12.20%,and in control group they are 87.80%,9.76%,2.44%.The x2 test demonstrate that the genetype distributions are statistically different between the CI and control groups (χ~2 =10.212,P=0.001<0.05). The C,T allele frequency of IL-lα(-889C/T) are 71.95%,28.05% in CI group,and 92.62%,7.32% in control group.The x2 value is 12.106 and the P value is 0.001<0.05,there is statistically different between the CI and control groups.3 The genetype distribution and allele frequency of IL-lβ(+3953C/T) and IL-lβ(-5llC/T) are not different in statistics between the two group(P>0.05).4 The genetype distribution and allele frequency of IL-4(-590C/T),IL-4(+33C/T) are not statistically significant between them (P>0.05).5 When taking high blood pressure,diabetes,hyperlipemia,smoking,alcohol abuse and the genetype of IL-lα(-889C/T)'s CC,CT,TT into considera -tion,the logistic regression analysis showed that TT genetype and high blood pressure,smoking all maybe have correlation with the cerbral infarction disease.Conclusions:1 Cerbral infarction is possibly an compli -cated gene heritage disease which is controlled by lots of factors. 2 The genetype distribution and allele frequency of IL-Iα(-889C/T) may be associated with the cerbral infarction disease in Shi Jia zhuang region.The people who have IL-Iα(-889C/T) TT genetype may have more chance to suffer from the cerbral infarction disease. 3 There may be not association between genetype distribution and allele frequency of IL-lβ(-511C/T),IL-lβ(+3953C/T) with the cerbral infarction disease in Shi Jia zhuang region.4 The genetype distribution and allele frequency of IL-4(-590C/T),IL-4(+33C/T)are uncorrelated with hereditary susceptibility of the cerbral infarction of Shi Jia zhuang region.