| Objective: Ulcerative Colitis (UC) is a kind of undefined chronic inflammatory disease of rectum and colon.The affection mainly on the mucous and submucous layer of colitis.mainly showing inflammation and ulcer first invation rectum and distal colitis.Major symptom are diarrhea.pus-bloody stool abdominalgia and tenesmus. It will Persist and outbreak again and again.Western medicinal therapy has unsatisfied effect.Traditional medicine has abundant experience to cure this disease.professor Li Dian Gui put forward a new academic viewpoint of Zhuodu. We also found out the main disease mechanism of UC was Pi weakness, chronic accumulation of Tanshi and further turning Shire so that we made up a therapy principle of healthiness spleen,dispelling damp and disappearing carbuncle as well as a prescription of Jie Du Xiao Yong prescription (JDXY). JDXY is a experiential prescription of professor Li Dian Gui. Prevenient animal experiment show JDXY can observably ant-inflammate and ease Pain.This experiment is to study JDXY'effect to rat and JDXY inefetion to Proinflammtory cytokines tumor neerosis factor alPha(TNF-α) and IL-1βof rats with uleer ative colitis(UC),discuss JDXY ant-inflammatal elements,Provid eacademic sustain to cure Ulcerative Colitis.Methods:60 SD female rat are randomly divided into 6 groups. Each group included 10 rats respectively. Execpt normal group every rat replicated UC model By TNBS/ethanol.The model group fill with sodium ehloride l0 ml everyday , Rats in SASP group were Perfused with 0.5g/kg SASP after model ,in the lagre Dose JDXY treatment groups fill with JDXY 18.3g/kg,the Middle-dose JDXY treatment groups fill with JDXY 9.2g/kg the small dose JDXY treatment groups fill with JDXY 4.6g/kg,last 10 days. Obsevre rat weight,stool every day, to 11 Day get rat'colon to observe inflammation degree,wound depth etc.The number of ulcer were calculated, the area of ulcer were masured and the index of injury were calculated with macrscopic observation.The degrees of inflammation,hyPeremia, and edema in colonic mucosa were graded with macrscopic observation.To exam TNF-α,IL-1βand analyse data by SPSS 11.5 Sotfware.Results:1 The rat'weight descend,stool is watery,and the rat'can be observed the changes such as emaciation,lazniss ,no luster in the hair,poor appetite and rat'colon has dieffrent degree damnification.2 There were several ulcers in the colonic mucosa of model control (MC) group,and the degrees of inflammation were serious. The number of ulcer on the colonic mucosa of JDXY medium dose (JDXYm) group and JDXY high dose (JDXYh) group and SASP group were0.8±0.79,1.5±0.53 and 1.6±0.70 ,They were significantly decreased compared to the MC group2.4±0.84 (p<0.05), JDXYh group and SASP group have remarkable difference,the differences have statistical meaning(p<0.05), JDXYm group and SASP group have no remarkable differences(p>0.05), JDXY small dose (JDXYs) group 2.1±0.74 and MC group have no remarkable differences(p>0.05). The area of ulcer of JDXYh group and JDXYm group and SASP group were 3.56±1.03mm2,7.73±2.37mm2 and 6.38±2.72mm2,they were significantly decreased compared to the MC group 12.91±2.48 mm2 (p<0.05), JDXYh group and SASP group have remarkable difference,the differences have statistical meaning(p<0.05), JDXYm group and SASP group have no remarkable differences(p>0.05), JDXY small dose (JDXYs) group 11.47±5.09 mm2 and MC group have no remarkable differences (p>0.05).The index of injury of JDXYh group and JDXYm group and SASP group were 1.6±0.52,2.8±0.79 and 2.3±0.82,they were significantly decreased compared to the MC group 3.9±0.88 (p<0.05), JDXYh group and SASP group have remarkable difference,the differences have statistical meaning (p<0.05), JDXYm group and SASP group have no remarkable differences(p>0.05), JDXY small dose (JDXYs) group 3.6±1.01 and MC group have no remarkable differences (p>0.05).The degrees of inflammation of JDXYm group and JDXYh group and SASP group were significantly decreased compared to the MC group (p<0.05). JDXYh group and SASP group have remarkable difference,the differences have statistical meaning (p<0.05), JDXYm group and SASP group have no remarkable differences(p>0.05), JDXY small dose (JDXYs) group and MC group have no remarkable differences(p>0.05).3 Contents of TNF-αand IL-1β:The Contents of TNF-α: The Contents of TNF-αin serum of MC group29.61±5.41 were significantly increased compared to the normal control group 16.28±1.99 (p<0.05), The Contents of TNF-αin serum of JDXYh group and JDXYm group and SASP group were 16.81±2.51,20.49±4.54 and 21.23±5.24 ,they were significantly decreased compared to the MC group (p<0.05), JDXYh group and SASP group have remarkable difference,the differences have statistical meaning (p<0.05), JDXYm group and SASP group have no remarkable differences(p>0.05), JDXY small dose (JDXYs) group 25.81±2.96 and MC group have no remarkable differences(p>0.05). The Contents of IL-1β: The Contents of IL-1βin serum of MC group12.34±1.21 were significantly increased compared to the normal control group 7.94±0.52 (p<0.05), The Contents of IL-1βin serum of JDXYh group and JDXYm group and SASP group were 7.93±0.54,9.84±1.35 and 10.50±1.39 ,they were significantly decreased compared to the MC group (p<0.05), JDXYh group and SASP group have remarkable difference,the differences have statistical meaning (p<0.05), JDXYm group and SASP group have no remarkable differences(p>0.05), JDXY small dose (JDXYs) group 12.04±1.14 and MC group have no remarkable differences(p>0.05).Conclusion:1 The rat model is successful. For the first time the study pointed out that Pi weakness, Shi gravity and Zhuoduneiyun were UC's mechanisms. We made up a therapy principle of improving Pi,dispeling Shi and disappearing carbuncle and JDXY.The study proved that JDXY had great protective effect of UC rats, which was according with the clinic research.2 The number and area of ulcer of model rats were significantly reduced with the administration of JDXY Phatological changes such as inflammatory cell infiltration and edema were imProved.3 JDXY can improve rat'symptom and can down- regulate Proinflammtory cytokines TNF-αand IL-1β,It may be ant- inflammate through down-regulate Proinflammtory cytokines TNF-αand IL-1β.
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