The Influence Of Pine-seed Oil To Fat Metabolism And Oxidation Resistance Function

Pinus koraiensis Sieb.et Zucc is a rare tree species of Xiao xing an ling in China. Pine seed oil includes many kinds of developed activity materials, can be edible or used in functionality food, so it has the broad development prospect.This paper respectively feed the mice by high fat feedstuff (80% added 20% lard HF) and added pine-seed oil feedstuff(80% high fat feedstuff added 20% pine-seed oil SHF), record weight to adjust the quantity of feeding, in 45 days and 90 days, measure the body fat content and the enzyme method measurement blood fat level, and also in 45 days and 90 days, takes the brain and liver to make the tissue homogenate and measures MDA,LP,CAT,SOD and GSH-PX level. Takes the fat of periphery testicle, assay ACS,FABP,FATP,HSL,and PPAR-γby RT-PCR. The main results are as follows:1,In the trial period, the energy taking of SHF experiment animal is obviously higher than the normal group and HF(P<0.05). Although when the experiment is over, the body weight of SHF is obviously higher than that of the normal group,but the body weight is more remarkable reducer than that of HF. So the results explained that the pine-seed oil can reduce the body weight and can reduces the body fat content.2,Pine seed oid can make the high fat small mice serum TG,TC, and LDL-C lower(P<0.01), but the HDL-C go up(P<0.01), suggested that pine-seed oil has definite effect on regulating blood fat and has positive significance in fat metabolism.3,The LPS activeness in brain and liver of SHF obviously elevate(P<0.01), so it prompts that the pine-seed oil can accelerate the fat disintegration rate in the brain and the liver by enhancing LPS activeness.4,The pine oil may simultaneously enhance ACSmRNA, FATPmRNA and the HSLmRNA expression, promote fat decomposition, reduce in vivo fat storing; simultaneously may reduce PPAR-γduplication activeness, suppress FABPmRNA the expression, then suppress the fat the production.5,Feeds high fat food to be possible to cause mice brain and liver CAT, total SOD, the GSH-PX activeness obviously reduces(P<0.01), and along with time each enzyme's activeness assumes the declining trend, simultaneously the brain and the liver's CAT and the LP content obviously elevate(P<0.01), prompt the high fat feed to accelerate in vivo lipin peroxidization advancement. Although the pine seed oil cannot reverse in vivo oxidation resistance enzyme activity and reducing the content of the lipin peroxide content by the high fat feed, but actually the pine seed oil may partly improve this kind of change, particularly CAT and the total SOD activeness obviously is higher than the normal group in the experiment intermediate stage and the last stage(P<0.01), prompts the pine seed oil to be possible through to enhance the antioxidase system's activeness to reduce in organism the free radical to respond, thus enhances organism oxidation resistance ability.