Study On Extraction And Sepatation Process Of Main Alkaloids In Camptotheca Acuminata

This study took Camptotheca acuminata(Nyssaceae) as material,an indigenous species of China,also pilot study on distribution of Camptothecin and 10-hydroxycamptothecin in it. Based on the study mentioned above,extraction from Camptothecin(CPT)and 10-hydrxycamptothecin(HCPT) in seeds and leaves of Camptotheca acuminata were optimized by response surface methodology(RSM).Optimum technology parameters on seeds that 50%of ethanol volume fraction,3min of homogenate time and 7(mL:g) of liquor to material,while, optimum technology paramers on leaves that 70%of ethanol volume fraction,6min of homogenate time and 11(mL:g) of liquor to material.Exploring purification process of Camptothecin as organic solvent extraction,liquid-liquid extraction,recrystallization etc,and determined the optimal conditions of the experiments,finally in pilot magnification,the purity of CPT in extracum is 98%and the extraction yield of cpt is 0.0216%.Optimized separating method of HCPT from the remainder extracts for the production of CPT with macroporous resins adsorption-desorption.X-5 resin offers the best adsorption and desorption capacities for HCPT and the optimum conditions for adsorption were that pH was 5,40℃of temperature, 4BV/h of flow rate and 5BV of sample processing volume.The optimum conditions for desorption were that 10-90%of ethanol were used as solvents for gradient elution,30-60%of ethanol eluates were collected as final product which was concentrated.The flow rate was 4BV/h and the volume of elution was 2BV.The mid-test results show that the content of HCPT in the product increased to 7.19%,and the recovery yield was 82.1%.Optimization of recrystallization to purified HCPT,and its purity achieved 95%.This thesis is obtained for materials on seeds and leaves of Camptotheca acuminata,at the same time producing CPT and HCPT on mid-test.This technology is lower production costs,higher rates and highly pure,and effective utilization of CPT and HCPT in Camptotheca acuminata has guiding significance.