Separation And Purification Of Alkaloids From The Puppies In Boluo

Macleaya cordata (Willd.) R.Br. is a species from genus Papaveraceae,Macleaya perennial herbaceous plants,an effective ingredient for the vision of alkaloids-isoquinoline, including benzophenanthridine?protopine?synonyms analogous alkaloids, Macleaya cordata(Willd.)R.Br.mainly including benzophenanthridine?protopine analogous alkaloids. Protopine analogous alkaloids including sanguinarine and chelerythrine, Protopine analogous alkaloids including protopine and allocrytopine. sanguinarine and chelerythrine has antibacterial and cancer of the pharmacological effects, Macleaya cordata Extractions contains both sanguinarine and chelerythrine is widely used in animal feed additives in western countries, enhance immunity and disease prevention. protopine. In this experiment, the study is about Macleaya cordata (Willd.) R.Br. To establish a HPLC method for the determination of protopine and allocrytopine contents in Macleaya cordata (willd.)R.Br, Compared Macleaya cordata (willd.)R.Br alkaloid content of different parts. Micolta Bioresource Inc. to mass produce and for the materials with by-products, protopine analogous alkaloids preparation technology, based on protopine analogous alkaloids extractions to separate protopine and allocrytopine. This production process will be the full utilization of resource for Macleaya cordata (Willd.) R.Br., reduce the pollution of the environment. Mainly made a few aspects of work:1. Determination of protopine and allocrytopine in different parts of Macleaya cordata (willd.)R.Br by HPLCEstablish HPLC method, for determination the protopine and allocrytopine contents of different parts of Macleaya cordata (willd.)R.Br, planting in Micolta Bioresource Inc planter.2. Study on enrichment process of Protopine alkaloids extractions by macroporous adsorptive resins methodsWith the by-products for the research object, used static adsorption-desorption as evaluation parameters, choose the most appropriate type of macroporous adsorption resin. Simultaneously optimize the process parameters.3. Study on preliminary separation process of protopine and allocrytopineThe study is about protopine analogous alkaloids extractions, used contents and transfer rates of protopine and allocrytopine as evaluation parameters, choose the best solvent for separate protopine and allocrytopine, and optimize process parameters.4. Study on refining process of protopineThe study is about protopine coarse products, used contents and transfer rates of protopine as evaluation parameters, choose the allocrytopine solvent systems for protopine, as the same time, optimize process parameters.5. Study on refining process of allocrytopineThe study is about allocrytopine coarse products, used contents and transfer rates of allocrytopine as evaluation parameters, choose the allocrytopine solvent systems for protopine, as the same time, optimize process parameters.Through the above work, this research has the following:1. Determinating the protopine and allocrytopine contents of different parts of Macleaya cordata (willd.)R.Br, alkaloids content of roots, leaves and fruits is higher. Considering the limited resources of the roots of Macleaya cor data (willd.)R.Br, and the vegetation destruction of dig. So the leaves and fruits of Macleaya cordata (willd.)R.Br will be suitable for produce protopine analogous alkaloids extractions.2. Comparing different types of macroporous adsorptive resin for protopine and allocrytopine static adsorption-desorption performance, summarizes production process of the protopine analogous alkaloids extractions.3. Establish and optimize solvent methods process, can effective separate protopine and allocrytopine.4. choose the best protopine crystallization solvent systems, and the optimization of process parameters, establish the 98% protopine optimum production process.5. Establish a simple, practical, safe, low cost refining process of allocrytopine.