| Objective: To explore the relationship between gene polymorphisms of IL-10 gene promoter -1082 , -592 and HBV carriers, chronic hepatitis B with mild, moderate and severe degree, cirrhosis, chronic serious hepatitis as well as the expression of HBeAg.Methods: The patients chosen as the researched subjects, with chronic hepatitis B from the First Affiliated Hospital of Guangxi Medical University, were in accordance with the diagnosis of virus hepatitis revised jointly by Infection Branch and Liver Disease Branch of Chinese Medical Association in 2000. In the chosen patients, there were 147 cases with chronic hepatitis B (including 35 cases with mild, 53 with moderate, and 59 with severe degree), 38 with hepatitis B virus carriers, 47 with cirrhosis, 34 with chronic serious hepatitis, and 35 health control. In addition, the subjects were divided into HBeAg positive and negative groups. In group with HBeAg positive, the patients with chronic hepatitis B, chronic serious hepatitis, cirrhosis, carriers were 74, 19, 14 and 17 cases respectively; In group with HBeAg negative, the subjects with chronic serious hepatitis, cirrhosis, carriers were 73, 15 , 33, 21 cases respectively. Liver function was detected by automatic biochemical analyzer. HBV markers in serum was tested by ELISA assay, and IL-10-592 and -1082 genotypes were detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis techniques. Results: (1) The distribution of -592 in various clinical types with liver diseases was that the frequencies of -592AA in chronic hepatitis B, chronic serious hepatitis, cirrhosis, carriers and normal control were51.7%(76/147), 58.8% (20/34), 38.3% (18/47), 31.6% (12/38) and 51.4% (18/35) respectively, and the frequencies of -592CC were13.6%(20/147), 8.8% (3/34), 21.3% (10/47), 36.8% (14/38) and 34.4% (12/35) respectively. The distribution of IL-10-592 genotype was significant different in the 5 groups above (P=0.003). The results of further comparison each other among the 5 groups were as follows:â‘ There was no significant statistical difference (P=0.127) in the frequencies of IL-10- 592AA/AC/CC genotype between carriers and the controls;â‘¡There was significant statistical difference on genotype CC at position -592 between chronic hepatitis B, chronic serious hepatitis, cirrhosis group and the normal group(P<0.05). The frequencies of 592CC in the 5 groups above were significantly lower than that of normal group (P=0.005,0.021,0.034 respectively);â‘¢The frequencies of -592CC in the 2 groups of chronic hepatitis B, chronic serious hepatitis were significantly lower than that of HBV carriers(P=0.003, 0.001 respctively).The frequencies of -592C allele in chronic hepatitis B, chronic serious hepatitis, cirrhosis, HBV carriers and normal control were 31%(91/294), 25% (17/68), 41.5% (39/94), 52.6% (40/76) and 41.4% (29/70) respectively; The distribution of IL-10-592 allele among the 5 groups above also showed significant difference (P=0.001). The results of further comparison each other among the 5 groups were as follows:â‘ There was no significant difference of the distribution in frequencies of -592A/C allele between HBV carriers and normal group(P=0.176);â‘¡There was significant difference of -592A/C allele frequencies between cirrhosis, chronic serious hepatitis and the normal group (P=0.000, 0.041);â‘¢T he distribution of -592C in cirrhosis group was much higher than that in chronic severe hepatitis group(P=0.029);â‘£Frequencies of C allele at position -592 in chronic hepatitis B, chronic severe hepatitis were much lower than that in asymptomatic HBV carrier group (P=0.000, 0.001); (2) The frequencies of -1082AA locus in chronic hepatitis B, chronic serious hepatitis, cirrhosis, HBV carrier and normal groups were80.3%(118/147), 52.9 (18/34), 85.1 (40/47), 50% (19/38) and 51.4% (18/35) respectively; The frequencies of -1082GG loci were 11.6%(17/147), 32.4% (11/34), 8.5% (4/47), 28.9% (11/38) and 31.4%(11/35) respectively; The frequencies of IL-10-1082A allele in the 5 groups above were84.4%(248/294), 60.3% (41/68), 88.3% (83/94), 60.5% (46/76) and 60% (42/70) respectively. The results showed that there was significant difference of the frequencies of IL-10-1082 locus genotypes and alleles between the 5 groups (P=0.000, 0.000). The results of further comparison each other among the 5 groups were as follows:â‘ IL-10-1082AA genotypes and A alleles frequence in chronic hepatitis B, cirrhosis were significantly higher than that of the normal group.(P=0.003,0.000; 0.004, 0.000).;â‘¡There was no significant difference of the frequencies of IL-10-1082 genotypes and alleles between chronic hepatitis B, cirrhosis and the carrier group (P=0.001,0.000, 0.002, 0.000). The frequencies of -1082AA/A in the 2 groups above were significantly higher than that of carrier group (P<0.05);â‘¢IL-10-1082AA genotype and A allele frequencies in chronic hepatitis B and cirrhosis were much higher than that in chronic severe hepatitis group (P=0.005,0.000, 0.006, 0.000). The frequencies of -1082AA/A in the 4 groups above were significantly higher than that of chronic serious hepatitis (P<0.05). (3) The relationship between -592 loci and HBeAg was that the frequencies of IL-10-592AA genotype and -592A allele in cirrhosis with HBeAg positive were significantly lower than that in HBeAg negative groups (P=0.010, 0.003). (4) IL-10-1082 locus genotypes and-1082A/G allele frequency distribution in group of chronic serious hepatitis with HBeAg positive was higher than that in group with HBeAg negative (P=0.019, 0.000). The frequency of -592AA/A in group with HBeAg positive was higher than that in group with HBeAg negative (P <0.05). (5) The frequency of -1082A allele in cirrhosis with HBeAg negative was significantly higher than that in group with HBeAg positive (P=0.024).Conclusions: (1) -592CC genotype and -592A/C allele polymorphism are related to the different clinical types of liver diseases with HBV infection in the Guangxi crowd. (2) There is significant relaionship between IL-10-1082 AA, IL-10-1082 A/G and the persistent of HBV infection in different clinical types of liver diseases in the Guangxi crowd. (3) IL-10-592AA genotype and-592A/C allele may be associated with the expression of HBeAg in cirrhosis patients. (4) IL-10-1082 AA locus genotypes and -1082A/G alleles may be related to the expression of HBeAg in chronic serious hepatitis patients. (5) Perhapers -1082A allele is correlated to the expression of HBeAg in cirrhosis patients.
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