Parenteral Nutrition Leads To Alteration Of Intestinal Inflammatory Cytokines & Receptors Gene Expression In Rats

Objective:Total parenteral nutrition(TPN) is a life-saving therapy for patients with intestinal failure.Raise the survival rate of child and adult.Althougn the technique of infusion and compounding is improved,prolonged parenteral nutrition is also associated with complications.Long-term parenteral nutrition induces a high rate of mucous membrance of small intestine atrophy,gastrointestinal immune dysfunction,bacterial translocation,even to lead to intestinal source septicaemia.Although many pathogenesis have been suggested to be responsible for it,the pathophysiology is still unclear.The aims of this research study were to identify Inflammatory Cytokines & Receptors Gene which may be either increased or decreased in expression during the administration of TPN with a real time PCR array.Material and methods:12 male SD rats,weighting 225～250g,special pathogen free,were housed in metabolic cages and subjected to an acclimatization period of a week. They were fed standard rat chow and had access to tap water ad labium.Rats were maintained under a 12-hr light-dark cycle at a temperature of 22℃and a relative humidity of 40%to 60%.The rats were randomized into two groups,The control group(N=6) and the TPN group(N=6).Total parenteral nutrition group received continuous TPN infusion through a silastic catheter inserted in the right jugular vein and the control group infusion the same physiologic saline by the same way.After 7 days,we used real time PCR array to analyze the differential expression of the cycle genes between the two groups.Results:(1).Two groups were found to have gained weight on the last experimental day compared with the beginning of the experiment.The weights in the control group were higher than those in the TPN group.(2).Control group tissue of intestine was not found change at the electron microscope,but the TPN group was found the atrophy of microvillus,engorgement of mitochondria,cell-cell junction breakage and several apoptotic body.(3).The up-regulation Inflammatory Cytokines & Receptors genes of TPN group are IFN-γ,IL1R1,IL8Rb.The down regulation Inflammatory Cytokines & Receptors genes of TPN group are CCL17,CCL19,CCL21,CCL22,CCL9,CXCR3,CCR3,CCR7,CCR5,CRP,IL-10.Conclusion:TPN lead to several significant alternations of Intestinal Inflammatory Cytokines & Receptors gene,such as cytokine IFN-γincrease and IL-10 decline. Chemokine and receptors family CCL19,CCL21,CXCR3,CCR3,CCR7 and CCR5 decline too.The alterations of these gene may be directed associated with the development of intestinal immune and mechanical barrier dysfunction.