Inflammatory Cytokines And Receptors Gene Expression Of The Liver From Total Parenteral Nutrition-Associated Hepatic Dysfunction Rats

Objective: total parenteral nutrition has been widely used during the past thirties years. It has been found to be an effective and relatively safe method for supplying energy and nutrients to patients with intestinal dysfunction, however, long-term TPN induces a high rate of liver disease in infants. The pathophysiology of parenteral nutrition–associated hepatic dysfunction in young children is poorly understood although it is almost certainly multifactorial in origin .The goal of this experiment was to further studied the roal of inflammation cytokines and receptors and the potential mechanism of TPN-induced hepatocyte injury by investigating the differential gene expresision of the inflammatory cytokine and receptors.Material and methods: Twelve infant (22～28 days old ) male SD rats weighing 90～100g were randomly allocated two groups (n=6), control group received an oral diet, total parenteral nutrition group received continuous TPN infusion through a silastic catheter inserted in the right jugular vein. After 4 days part of the liver was taken for the examination of inflammatory cytokines and receptors gene expression with the oligo gene microarry to analyse the differential expression of the genes in the rats accepted TPN infusion.Results:(1)Two groups were found to have gained weight on the last experimental day compared with the beginning of the experiment. The body weights in the control group were higher than those in the TPN group(.2)The TPN group was found to have large lipid droplets with unclear margins in the perilobular region and smaller lipid droplets in the centrilobular regions.(3) Comparing the expression of the inflammatory cytokines and receptors'gene of the control and TPN group. TPN group was associated with an up-regulation of IL1r2 IL-1βIL1rn IL-18 TGF-αTGF-β1 TGF-β2 TGF-β3 TGF-β1il TNF-sf6 IFN-γbp Ccl4 Cyrl. down-regulation of Bmp2 XCR1 FGF10 FGFr2 IL-15 TGF-βr2 Scya11 FGF1 IL-10αLTa Bambi Caspase1 Cxcl10 FGF16 FGF21 Frag1 TNFsf4.Conclusion: TPN lead to significant alternations in the gene expressions of the inflammatory cytokines and receptors of the liver, which may give insight into the potential mechanism of TPN-induced liver injury.