The Study Of Effects Of Jianpi Shugan Jiedu Recipe On The Expression Of P53 And Survivin Gene During DEN-Induced Hepatocarcinogenesis In Rat

Objective:to observe The Effects of Jianpi Shugan Jiedu Recip on the Expression of p53 and survivin gene During DEN-Induced Hepatocarcinogenesis in Ra,and further explore its mechanism,with a view to effective clinical use of the JPSGJD recipe detoxification of liver cancer,especially cancer of advanced treatment provide an objective basis.Methods:male healthy rat weighting 110±10g,(n=72).feeding 3 days in normal. Later,rat were randomly divided into 6 groups by SPSS:DEN Control group, Middle-dose of JPSGJD with DEN group,High dose of JPSGJD with DEN group; BINZHENGJIEH modle Control group of JPSGJD,Middle dose of JPSGJD group, High dose of JPSGJD group.the chemotherapy drug was given by intraperitoneal injection for all group to the end.DEN SolutionConcentration is 1mlDEN:10000ml pured Sterile water,the rat drank that Solution everyday,until the end of the experiment.To establishe the DEN-Induced Hepatocarcinogenesis in Rat model. Secondly,we give traditional Chinese medicine DAHUANGConcentrated liquid Ig for BINZHENGJIEH modle Control group's all rat,at the same time,Long-term constraint rat limbs,Restraint rat' food,activtation to anger them.Throught this way to establish PIXUGANYU model on Rat.the dosage of Middle dose of JPSGJD group and High dose of JPSGJD group isl0mg/kg,30mg/kg.At the end of the experiments,the rat were killed by cervical dislocation,we weighed the tumor,the spleen,the liver.Calculated the rate of inhibitory effect of liver carcinoma and the index of the spleen,the liver.HE stain,light microscopy level of metastasis.We measured P53,Survivin expression of the rat through Immunohistochemistry technique method.To explore the mechanism of the JPSGJD recipe on the liver cancer of mechanism.Results:It has showed that the JPSGJD recipe has remarkably suppressing effects on cancer of the DEN-Induced Hepatocarcinogenesis in Rat.It can significantly increase the inhibition rate of DEN-Induced Hepatocarcinogenesis in rat of liver cancer(compared with the control group,P＜0.01);In addition,the JPSGJD recipe can significantly improve the mice's immunologic function,especially for High dose of the SGJPJD recipe(compared with the control group,P＜0.01).The JPSGJD recipe can decrease the expression of P53 and Survivin protein expression(compared with the control group,P＜0.01),and the JPSGJD recipe with DEN group which compared with the DEN group and model group could have statistic differences(P＜0.01).Conclusion:The JPSGJD recipe can inhibit liver cancer in rat,Their role may be related to P53,Survivin gene reduce expression;The JPSGJD recipe can also enviously enhance the immunologic function of liver cancer rat.