| Objective:1.To observe the effects of fluorine and combination of aluminum and fluorine on body weight and organ coefficient of rats.2.To study the effects of fluorine and combination of aluminum and fluorine on index of blood routine and blood biochemistry of rats.3.To understand the effects of chronic fluorosis on renal function of rat and the relationship between oxidative stress of kidney.4.To study the effects of chronic fluorosis on morphologic changes of liver and kidney of rats.Methods:56 Wistar rats were randomly divided into 7 groups,and exposed to a series of doses of fluoride and aluminum(F+Al),which were(0+0),(100+0), (200+0),(300+0),(100+10),(200+10),and(300+10) mg/L.After 96 days intragastric administration,blood samples were collected on eyes of rats to determined blood routine:red blood cell(RBC),white blood cell(WBC),lymphocyte(LYM),platelet (PLT),hemoglobin(HGB),hematocrit(HCT),mean corpuscular hemoglobin(MCH), Mean corpuscular-hemoglobin concentration(MCHC),mean corpuscular volume (MCV),at the same time some blood biochemistry indicators related to functions of liver and kidney were determined such as aspartic acid aminotransferase(AST), alanine aminotransferase(ALT),alkaline phosphatase(ALP),Crea(Cr) and Urea. Organ coefficient of liver and kidney were calculated.The kidney homogenates were prepared to detect the content of malondialdehyde(MDA),nitric oxide(NO) and the activity of catalase(CAT),glutathione peroxidase(GSH-Px) and nitric oxide synthase (NOS).The tissues of livers and kidneys in experimental rats were fixed by formaldehyde after washing away the blood stains,and were made into conventional slice with HE staining.Pathological changes of rat kidney and liver were observed by light microscopy.Results:1.The body weight of rats are increasing with experimental time,while the control group and relapse group no significant difference.There is interaction between treating effectors and experimental time.2.Compared with the control group, the RBC of exposed groups obviously increased while the MCV remarkably diminished(P＜0.05),AST and Urea also significantly increased(P＜0.05 or P＜0.01). At the same time,the part of data under this experimental conditions suggest that there was a trend about the joint role of aluminum and fluoride:aluminum appears synergistic effect in the low-dose fluoride group while has antagonistic joint action in the high-dose group.3.Compared with the control group,the Urea of exposed groups obviously increased(P＜0.05).The activity of CAT,GSH-Px,NOS depressed,and the content of NO decreased while the content of MDA increased.There was a significant difference(P＜0.05 or P＜0.01).4.We can see by light microscope that the contour of hepatic lobule is unclear,the cells of sinus tissue are mild hyperplasia,and the central vein and hepatic sinusoid are mildly congestive and expanded.The epithelial cells of kidney proximal tubule are granular degeneration in varying degrees,some are vacuolar degeneration,and interstitial tubule are mildly congestive and expanded.Conclusion:1.The effects of and combination of aluminum and fluorine on body weight of rats are not significant,but they affect on organ coefficient of liver and kidney of rats to some extent.2.Fluorine and combination of aluminum and fluorine have toxicity on rats to a certain extent.The erythrocyte of rat are proliferated,while the cell size is made small and the cell quality is deteriorated,meanwhile functions of liver and kidney are impaired.Aluminum shows different joint action in different concentrations of fluorine.3.Chronic fluorosis have toxicity on renal function to a certain extent,and the increasing of oxidative stress and inhibition of antioxidant enzymes may be one of the mechanisms caused by fluoride in kidney tissue injury of rats.4.Chronic fluorosis have a certain morphological influence on liver and kidney of rats.
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