The Expression Of MMP-2 And TIMP-2 In Cervical Cancer And Its Significance

Objective:Cervical cancer is a most common malignancy in gynecologic tumor,ranked second among in the world of women malignant tumors.Matrix metalloproteinases (MMPs) are more important family of proteolytic enzymes,which are capable of degradation of the proteins composing the extracellular matrix and basement membrane.Tissue inhibitors of metalloproteinase(TIMPs) are MMPs' natural inhibitors.We aim to determine the expression of matrix metalloproteinase -2(MMP-2) and tissue inhibitor of metalloproteinase-2(TIMP-2) in cervical cancer and to study the relationship of their expression with clinicpathological characteristics and to demonstrate the potential use of them as a prognostic and therapeutic agent.Methods:Using streptavidin-biotin-protein-linked peroxidase method(ie,SP) immunohistochemical method to detect the expression of MMP-2 and TIMP-2 in 20 cases of normal cervical tissue,21 cases of CIN tissue,59 cases of cervical cancer. Analysis of cervical cancer in MMP-2 and TIMP-2 expression and clinical stage, histological grade and other clinicopathological parameters of relevance,at the same time the relationship between MMP-2 and TIMP-2 was studyed in the development of cervical cancer for a preliminary study.Results:1.Positive expression rate of MMP-2 in normal cervical tissues,CIN,cervical cancer tissues were 15.0%,38.1%,80.0%,the trend has gradually increased(p＜0.05). Among them,significant difference was existed between normal cervical group and cancer group(p＜0.05),also between CIN group and cancer group(p＜0.05).There was no significant difference between normal cervical group and CIN group(p＞0.05).2.In cervical cancer,positive rate of MMP-2 protein's expression at FIGO stagesⅡb～Ⅳperiod was higher than theⅠ～Ⅱa period(χ~2=5.848,p＜0.05), there is lymph node metastasis group was higher than that without lymph node metastasis group(χ~2=4.461,p＜0.05);Expression of MMP-2 in cervical cancer were not related with age,gross type,the degree of differentiation,histological types(p＞0.05).3.Positive expression rate of TIMP-2 in normal cervical tissues,CIN,cervical cancer tissues were 10.0%,33.3%,57.6%,the trend has gradually increased(p＜0.05). Among them,significant difference was existed between normal cervical group and cancer group(p＜0.05),also between CIN group and cancer group(p＜0.05).There was no significant difference between normal cervical group and CIN group(p＞0.05).4.In cervical cancer,positive rate of TIMP-2 protein's expression at FIGO stagesⅡb～Ⅳperiod was lower than theⅠ～Ⅱa period(χ~2=4.544,p＜0.05),there is bad differentiated group was lower than that well differentiated group(χ~2=5.281,p＜0.05),there is lymph node metastasis group was lower than that without lymph node metastasis group(χ~2=16.368,p＜0.05);Expression of TIMP-2 in cervical cancer were not related with age,gross type and histological types(p＞0.05).5.The relationship between MMP-2 and TIMP-2 was negatively correlated(r_s =-0.332,p＜0.05).Conclusions:1.The expression of MMP-2 and TIMP-2 in cervical cancer was higher than that in normal cervical tissues and CIN.It suggested that expression of MMP-2 and TIMP-2 may play a role in the carcinogenesis of cervical cancer.2.The expression of MMP-2 and TIMP-2 was correlated with FIGO stage and lymph node metastasis,and the expression of TIMP-2 was also correlated with histological grade,It suggested that expression of MMP-2 and TIMP-2 may associated with invasion and metastasis of cervical cancer.3.In cervical cancer group,expression of MMP-2 had a negatively significant correlation with expression of TIMP-2.MMP -2/TIMP -2 may be used as an important index to indicate the prognosis of cervical carcinoma.