Expression And Clinical Significance Of Nav1.7 In Cervical Cancer

Objectivesodium channels are the cell membrane structures formating by polymerizational proteins,which contains hydrophilic pore for sodium ion getting through.Cells are depolarized and hyperpolarized by controlling sodium ion outflow or infkow,then complete the transmission through the membrane.Retain the balance of body environment.they have massive expressions in many tumor cell.The aim of this thesis was to detect the expression of Na_v1.7mRNA in order to find their relation with cervical cancer and the prognosis by RT-PCR.Materials and MethodsMaterial:Tissue used in this study were obtaines from 50patients with invasive cervical cancer who underwent biopsy or curative resection,and another 10 pairs cervical cancer specimens from the same patients who before and after radioche motherapy at the First Affiliated Hospital of China Medical University.Besides,we collected 10 cases of normal cervical tissue.These tissue samples were obtained after hysterectomy because of benign uterine myoma.We collected the clinical information including pathological grades,clinical stages,age,before and after radiochemotherapy for statistical analysis.Main agents:RNAiso Reagent,TaKaRa RNA PCR Kit(AMV)Ver3.0,Forward and reverse primer of Na_v1.7 andβ-actin,the necessary agents of agarose gel-electrophoresis.Method:RT-PCRResult judgement:Na_v1.7mRNA amplified by PCR,agarose gelelectrophoresis, UV lamps observe results,and do semi-quantitative analysis by measuring the gray level.Statistical method:all data processed by SPSS13.0,measurement data was carried out by T-test.Differences were considered significant when P values were less than 0.05.Result1,The expression of Na_v1.7 mRNA in normal cervix and cervical cancerthe ratio of gray level of Na_v1.7 mRNA was1.6403 in cervical cancer,but no expression in normal cervix,there were significant differences.2,Correlation between the expression of Na_v1.7 mRNA and clinicopathologic materials in patients with cervical cancerThe ratio of gray level of Na_v1.7 mRNA was 1.9014 in the poor differentiation group,higher than that of the middle and high differentiation group 0.9601,0.9918 (P＜0.05).the ratio of gray level of Na_v1.7 mRNA was 0.9867 in stagesⅠ-Ⅱ,1.8993 in stagesⅢ-Ⅳ,there were significant differences(P＜0.05).The expression of Na_v1.7mRNA have no significant relation with age(P＞0.05).3,The expression of Na_v1.7 mRNA in the same patient with cervical cancer before and after radio-chemotherapyNa_v1.7 mRNA was1.4010 before radiotherapy and chemotherapy,0.3001 after radiotherapy and chemotherapy,they have significant differences(P＜0.01).Conclusion1,Na_v1.7 mRNA participate in the proliferation of cervical cancer cell.2,Na_v1.7 mRNA have a positive correlation with clinical stages and pathological grades,so they may be correlated with the malignant progression and prognosis of cervical cancer.3,The expression of Na_v1.7 mRNA in cervical cancer decrease after radiotherapy and chemotherapy.