Expression Of Nasal Epithelial TSLP In A Mouse Model Of Allergic Rhinitis And Its Influence Factors

Objective We administer the anti-mOX40 Ligand(OX40L),interleukin-4(IL-4) and tumor necrosis factory-α(TNF-α)in the BALB/c mice during the sensitization period of allergic rhinitis to investigate the role of Thymic stromal lymphopoietin(TSLP) in the pathogenesis of allergic rhinitis and its influence factors.Methods Twelve female BALB/c mice(6-Sweeks,weight18-20g) were randomly divided into two groups:normal saline control was group A(n=6 ),allergic rhinitis was group B(n=6 ).Mice of group B was sensitized by i.p.injection of 20μg ovalbumin(OVA) 0.3AL(OH)₃ ml saline soluble 1.0ml on day 0,2,4,6,8,10,14.Mice of group A was injected with the same dose of control saline at the same time.On days16-25,mice of group B was administered 2%OVA saline soluble 20μl intranasally,Saline was administered to the mice of group A in a similar manner.24h after the final antigen challenge,mice were killed,and serum was collected from all of each group for IgE,IL-4,IL-5,IL-13,and TNF-αin serum measured by ELISA. Noses were removed from mice.Half of noses were stained by immunohistocemistry to show the expression of TSLP in nasnal epithelial.Half of noses were extracted to RNA and reverse transcriptased to eDNA then expression of TSLP mRNA by Real-time quantitative PCR.Twelve female BALB/c mice(6-Sweeks,weightl8-20g) were randomly divided into two groups:anti-mOX40L was group C(n=6 ),control mouse IgG2a was group D(n= 6 ).Mice of group C,D were administered with OVA in the same manner to group B. Mice of group C were injected with 300 ug anti-mOX40L on day 0,3,6,9.Mice of group D were injectde with the same dose of control mouse IgG2a at the same time. 24h after the final antigen challenge,mice were killed,and serum was collected from all of each group for IL-4,IL-5,IL-13,and TNF-αin serum measured by ELISA.Noses were removed from mice.Half of noses were stained by immunohistocemistry to show the expression of TSLP in nasnal epithelial.Half of noses were extracted to RNA and reverse transcriptased to cDNA then expression of TSLP mRNA by Real-time quantitative PCR.Twenty-four female BALB/c mice(6-8weeks,weight 18-20g) were randomly divided into four groups:anti-mOX40L+mIL-4 was group E(n=6),anti-mOX40L+mTNF-αwas group F(n=6),anti-mOX40L+mIL-4+mTNF-αwas group G(n=6),anti-mOX 40L+control saline was group H(n=6).Mice of group E,F,G,H was administered OVA and anti-mOX40L as group C,and then mice of group E was administered mIL-4 intranasally on days 16-25,mTNF-αwas administered to the mice of group F at the same time,both of mlL-4 and mTNF-αwere administered to the mice of group G.Control saline was administered to the mice of group G.24h after the final antigen challenge,mice were killed.Noses were removed from mice.Half of noses were stained by immunohistocemistry to show the expression of TSLP in nasnal epithelial. Half of noses were extracted to RNA and reverse transcriptased to cDNA then expression of TSLP mRNA by Real-time quantitative PCR.Results Sensitization with OVA in BALB/c mice expressed TSLP in nasal epithelium in a mouse model of allergic rhinitis and elicited a strong Th2 response. including interleukin(IL)-4 and TNF-αproduction after antigen challenge.Expression of TSLP was down-regulated after injecting anti-mOX40L at the same time.But expression of TSLP was up-regulated when add IL-4,TNF-α,or both of them intranasally.Conclusion Expression of Nasal epithelial TSLP play a key role in a mouse model of allergic rhinits.Blockade of OX40L inhibits Th2 inflammation in allergic rhinitis. Th2 cytokines and TNF-αinfluence the expression level of TSLP.