Tissue Transglutaminase Inhibition Promotes Cell Death And Chemosensitivity In Ovarian Cancer

Objective: To investigate whether tissue transglutaminase (tTG) inhibitor monodansylca- daverine (MDC) promotes cell death and sensitivity of ovarian cancer cell line SKOV-3 to Paclitaxel chemotherapy in vitro.Methods: Ovarian cancer cell line SKOV-3 was selected. The expression of tTG was detected by immunofluorescence technology and reverse transcription polymerase chain reaction (RT-PCR). Based on cell culture, the influence of gradually increase doses and time of MDC on survival in SKOV-3 cell were detected by using MTT.The effect of MDC on apoptosis and sensitivity of SKOV-3 cell to Paclitaxel chemotherapy in vitro were measured by Flow cytometry (FCM).Results: Statistical analysis showed differences between the vehicle only–treated controls and the MDC-treated groups (P < 0.05). Meanwhile, apoptotic percent of MDC+ Paclitaxel groups were significantly higher (P<0.001) compared to the Paclitaxel groups. MDC -induced apoptosis in SKOV-3 cell line depended on the time and doses of MDC used.Conclusion: Tissue transglutaminase has some effect on growth of ovarian cancer cell, and suppressing its activty can promote cell death and sensitivity of ovarian cancer cell to Paclitaxel chemotherapy in vitro.