| Objective: Atrophic gastritis is a common and frequently -occurring disease of digestive system. For the past few years,many scholars have studied and investigated on it. They have made great contribution to the Diagnosis and therapy of atrophic gastritis . However, this disease is still short of effective and little-side-effect of Western medicine. Chinese medicine and differential treatment of this disease has a unique advantage.JieduHuoxue Prescription is composed of herba, fructus,goldthread,radix,Szechwan lovage rhizome,salviae miltiorrhizae, et al. In traditional Chinese medicine, it has the effects to detoxify, promote blood flow,and regulate qi to alleviate pain. For clinical application side,it has achieved remarkable curative effect. This research applies four-factor method composed of MNNG, sodium salicylate,hot saline,and irregular diet to duplicate the model of atrophic gastritis rats by intragastric administration. And we selected weimeisu,39 Wei-tai and Jiedu Huoxue for the prevention and treatment.Then discuss the functional mechanism of Jieduhuoxue Prescription curing AG from the perspective on adjustment of gastrointestinal peptide. Methods:70 healthy wistar rats,half male and half female, were randomly divided into 5 groups, 14 rats every group, named normal group, model group, Jiedu Huoxue Prescription group,sanjiuweitai group,weimeisu group.Model preparation: 160μg/ml MNNG freedom drinking, 2% sodium salicylates and 15% NACL solution freely (heating to 55℃) fills the stomach full alternately every other day, irregular diet(Overeating for two days, starvation one day). Normal group: Gives the intragastric administration of distilled water( 2ml/only/day) for comparison, the foundation feed feeds for 12 weeks.Model group: Makes the mold according to the model preparation method. Jiedu Huoxue Prescription group: Makes mold's at the same time, gives intragastric administration by the 51.7g/kg suspension one time daily.Sanjiuweitai group: Makes mold's at the same time, gives intragastric administration by the 10g/kg suspension one time daily.Weimeisu group: Makes mold's at the same time, gives intragastric administration by the 0.99g/kg suspension one time daily. After 12 weekends,we draw each 2(female and male each 1) from normal group and model group at random , and then we kill them , open the abdomen,take the stomach, especially we take each 1 tissue from sinus ventriculi and corpus ventriculi.Later we makes the light microscope observation fixedly with 10% formalin. Once the light microscope observation discovered the atrophy in big mouse's glandular organ of gastric mucosa , we finished making the mold. After the last time administration, we stopped giving the rats food and drink for 24(gives water) hours. Put them to death by decapitation and obtained blood 2ml. Added 7.5% EDTA-2Na 30μl and aprotinin 40μl to the blood and jolted in plastic tube. Centrifuged the blood (4℃3000rpm 10 minutes )and separated plasma to examine the content of MTL later. Put them to death by decapitation and obtained blood 2ml. Centrifuged the blood (4℃3000rpm 10 minutes )and separated serum to examine the content of GAS later.We cut the belly open to obtain stomach by aseptic technique, then sheared the stomach along arcus major ventriculi, rinsed gastric contents with ice-physiological saline, blotted it with filter paper and macroscopic observed it. At last, two pieces of tissues of sinus ventriculi were scissorsed, each piece about 1mm3. The tissues were fixed with 4 percent paraform, dehydrolysised with alcohol, paraffin imbedding, and cut into sheets. And the sheets were dyed to observe pathological changes of gastric mucosa with light microscope. All the data were dealt with satistical software SPSS13.0 And the data were in the presentation of x±s.The mean of each group were compared by using one-way ANOVA,showing the significant difference between the level of 0.05,0.01 as the standard.Results:1 General situation of rats in each groupNormal group:rats looked easy, quick action, shiny hair next to the skin, a larger appetite and stable, granular stool, light red colored tail; Model group:dispirited, easily frightened, move slowly, enjoyed twisting together with each other, loss of appetite, food was decreased ,diarrhea often, ears and tail were red purple color, weight gained slowy and thin physique.Other three control groups after treatment administration, the general situation of a marked improvement, weight gained,the situation of the activity, feeding, hair, stool characteristics,were improved with the drug delivery. The appetite of Jiedu Huoxue Prescription were more than other control groups. During the experiment, 2 rats were dead in each group: Jiedu Huoxue Prescription group, sanjiuweitai group ,weimeisu group.The reasons may be intragastric administration and drug purification and so on.2 The effect of Jiedu Huoxue Prescription on pathological morphology of gastric mucosa of atrophic gastritis rats2.1 Gross observationNormal group:Gastric mucosa were pink, shiny, covered with more mucus.And the surface of the mucosal folds were smooth and flexible which taken the rule shape. Model group:Pale gastric mucosa thinned.Furthermore,the submucosal vessel could be seen easily. The mucosal folds were level or taken the disorder shape. And the elasticity of stomach wall decreased Sanjiu weitai and weimeisu control group: The gastric mucosa thinned,to a greater or lesser degree, which were pale. And the elasticity of stomach wall decreased,to different extent.But the representation were better than other control group. Jiedu Huoxue Prescription group showed no significant difference between the normal control group, which was significantly superior to other control groups, to the naked eye observation.2.2 Optical microscope (HE staining)Normal group: Mucosal epidermis of rats was complete and the glandular organs array regularly. Lamina muscularis mucosae were thin.Model group: Mucosal epidermis of rats was complete and thin,of which the glandular organs array irregularly. Especially the glandular organs shrink back or disappear to different degree. And the mucous membrane tunica muscularis layer thickness were thickened with the mucous membrane extending to tunica propria. gastric mucosa Jiedu Huoxue Prescription: The mucosal epidermis was complete. The layer thickness of the gastric mucosa were even.And the glandular organs array regularly . Sanjiu weitai control group: Mucosal muscularis were relatively thin. Not very few glandular organs shrink back. Weimeisu control group: Mucosal muscularis layer thickness were obviously thickened.Many glandular organs shrink back.3 The effect of Jiedu Huoxue Prescription on MTL in blood plasma of atrophic gastritis ratsCompared with normal group, the content of MTL in model group was lower, which had statistical significance (P<0.01); Compared with model group, the contents of MTL in Jiedu Huoxue Prescription group, sanjiu group and weimeisu group were higher, which had statistical significance (P<0.01 ); Compared with Jiedu Huoxue Prescription group, the contents of MTL in sanjiu group and weimeisu group were lower (P<0.01).4 The effect of Jiedu Huoxue Prescription on GAS in blood serum of atrophic gastritis rats:Compared with normal group, the content of GAS in model group was lower, which had statistical significance (P<0.01); Compared with model group, the contents of GAS in Jiedu Huoxue Prescription group, sanjiu group and weimeisu group were higher, which had statistical significance (P<0.01 ); Compared with Jiedu Huoxue Prescription group, the contents of GAS in sanjiu group and weimeisu group were lower (P<0.01).Conclusion:1 Jiedu Huoxue Prescription could improve the changes of pathomorphology in gastric mucosa, relieve the damnification of gastric mucosa. It has the function of protecting gastric mucosa of atrophic gastritis rats effectively.2 Jiedu Huoxue Prescription could increase the content of MTL in blood plasma, promote gastric motility .So it could be good for reparation of gastric mucosa.3 Jiedu Huoxue Prescription could increase the content of MTL in blood serum, promote gastric secretion and gastric motility.And it has the function of nourishing gastric mucosa.
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