Exploration The Association Of HLA-DRB1 Alleles With Systemic Sclerosis In Guangxi Zhuang And Han Nationality Patients

Objective:To explore the association of HLA-DRB1 alleles with systemic sclerosis(SSc) of Zhuang and Han nationality in Guangxi region.Methods:Polymerase Polymerase chain reaction-sequence specific primer technique(PCR-SSP) was used to study the HLA-DRB1 alleles in 58 Zhuang and 53 Han nationality patients with SSc.50 randomly selected,unreleated healthy controls of Zhuang and Han nationality respectively in Guangxi region.Results:1.The distribution of HLA-DRB1 alleles:(1) 14 alleles were observed in SSc group.In Zhuang nationality,the range of allelic frequencies was from 0.826%to26.724%.Among them the DRBI*1301,*1305and*15 allele were high(7.760%,11.207%,26.724%),the DRB1*04,*07,*10 were rare(0.826%,equally),DRB1*01 and *1402 have not been examined.In controls,the range of allelic frequencies was from 1.00%to 15.00%,the DRB1*01,*0301,*04,*1301,*1402 were rare(1.00%,equally).(2) 14 alleles were observed in SSc group.In Han nationality,the range of allelic frequencies was from 0.943%to28.302%.Among them the DRB1*1305and*15 allele were high(16.981%,28.302%),the DRB1*0301was rare,DRB1*01 and *07 have not been examined.In controls,the range of allelic frequencies was from 1.00%to 12.00%,the DRB1*01,*04,*08,*1001,*11*,1301,*1302,*1401,*1402 were rare(1.00%,equally).2.The comparison of HLA-DRB1 allelic frequency:(1) DRB1*1301,*1305,*15 allelic in the SSc group of Zhuang were significantly higher than that in the control group(7.760%VS1.00%,RR＝9.00,X~2＝4.341,P＝0.037;11.207%VS 4.00%,RR＝3.322,X~2＝4.206,P＝0.04; 26.724%VS15.00%,RR＝2.697,X~2＝6.038,P＝0.014)(2) DRB1*1305,*15 allelic in the SSc group of Han were significantly higher than that in the control group(16.981%VS5.00%,RR＝4.629,X~2＝8.518,P＝0.004;28.302%VS12%,RR＝4.130,X~2＝11.325,P＝0.001).(3)No significant difference of other allelic frequencies was observed between the two subjects.Conclusion:HLA-DRB1*15,*1305,*1301 were closely related to the invasion of SSc in Guangxi;HLA-DRB1*1305and*15may be the susceptible genes of SSc in Guangxi Zhuang and Han nationality,HLA-DRB1*1301 may be the variation gene of SSc in Guangxi Zhuang;The protecting gene was not found in Guangxi Zhuang and Han nationality.