The Detection And Clinical Significance Of CD4⁺CD25^HiCD127^Lo Regulatory T Cells In The Peripheral Blood Of Patients With Lung Cancer

Objective:To evaluate the expression and clinical significance of CD4⁺CD25^HiCD127^Lo regulatory T cells in the peripheral blood from patients with lung cancer in order to find more simple, expensiveless and accurate method to detect Treg cells in the peripheral blood of lung cancer patients. In the meanwhile, the relationship between regulatory T cells and lung cancer, and between regulatory T cells and IL-10 and TGF-β1 were also studied. The mechanism of regulatory T cells to suppress immune function were explored for a theoretical support to treat lung cancer patients with immune therapy.Methods:30 patients with lung cancer (20 of squamous cell carcinoma, 10 of malignant adenoma) and 20 healthy volunteers were included in this study . The proportion of CD4⁺CD25^HiCD127^Lo regulatory T cells in CD4⁺ T cells was evaluated by flow cytometry analysis.The serumlevel of IL-10 and TGF-β1 was measured by ELISA.Results:The proportion of CD4⁺CD25^HiCD127^Loregulatory T cells in patients with squamous cell carcinoma(n=20),malignant adenoma (n=10)were all significantly higher than that of healthy controls(P<0.05),but there weren't obvious differences among the two groups of different pathological type(P>0.05).The proportion of CD4⁺CD25+regulatory T cells in CD4 +T lymphocytes of peripheral blood in lung cancer patients was obviously higher than that of normal controls with significant difference(P<0.05). The ratio of CD4/CD8 of lung cancer group was lower than that of normal controls, but no significant was found(P>0.05). The serum level of IL-10 and TGF-β1 was also increased in lung cancer patients.Conclusion: The proportion of CD4⁺CD25^HiCD127^Lo regulatory T cells was significantly increased in lung cancer patients,which may consequently result in inhibitation of host immune response by excrete IL-10 and TGF-β1.