| Objective To investigate the changes of intermedin (IMD) and its receptors, calcitonin receptor-like receptor (CRLR) and receptor activity-modifying proteins (RAMPs), in rat kidneys after renal ischemia/reperfusion injury (IRI). It provides basis to further study about the function and mechanisms of vasoactive substance involving in renal IRI.Methods Sixteen male Wistar rats were randomly divided into sham-operated and IRI groups. IRI rats models were made by placing both renal arteries by non-traumatic vascular clamps for 45min. Both renal pedicles and arteries of sham-operated rats were just exposed but not clamped. Blood and kidney samples were harvested 12 hours after reperfusion. Renal function was observed by measuring serum creatinine (SCr) and blood urea nitrogen (BUN) concentration. The contents of IMD and ADM in plasma and renal tissue were assessed by radioimmunoassay (RIA). The mRNA expressions of IMD, ADM, CRLR and RAMPs in the kidneys were determined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR).Results SCr and BUN significantly increased in IRI rats compared with the sham ones. Serum and renal content of IMD in IRI group increased by 53.76% and 58.34% (P<0.05), consitent with the marked increase of serum ADM (increased by 143.22%, P<0.01). The mRNA expressions of IMD, ADM, CRLR, RAMP1,2,3 were up-regulated by 22.1%, 41.1%, 32.8%, 33.7%, 35.9%, 29.4% (P<0.05).Conclusions The amount of IMD and its receptors are up-regulated with renal IRI, it maybe compensatory change. It suggests that they may participate in the pathogenesis of renal IRI.
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