| Objective: To study the effect for oxymatrine to resist on the supernatant of the quartz to stimulating macrophage on signal protein of lung fibroblast in vitro. Methods: SD neonatal rats'lung fibroblasts were cultured and passed up to 4-5 generations and the experiment would be started. the lung fibroblasts were administrated by cell cultured the supernatant of the quartz stimulated macrophage and the supernatant combined with the oxymatrine.we observed the Hydroxyproline level in its supernatants, the expression of Gq, PLC,PKC protein of lung fibroblast with means of immunohistochemistry, the concentration of intracellular free Ca2+ with means of FCM in different time points. Results:1.When the quartz - macrophage culture supernatant acted on the lung fibroblasts, the level of hydroxyproline was obviously high in the supernatant, comparing with control group,statistical significance was considered at P<0.05.When the supernatant combined with different concentration OM, the level of hydroxyproline in the supernatant were be reduced, comparing with supernatant groups, statistical significance was considered at P<0.05. 2.The Gq protein results showed that: When the supernatant act on fibroblasts at different time points, the expression of Gq protein was obviously higher than the control group and the positive unit of Gq increased with the action time prolong, statistical significance was considered at P<0.05.When supernatant combined with OM to act on the lung fibroblast, the expression of Gq protein of lung fibroblast had no obvious difference comparing with the control group. 3.The PLC results showed that: when supernatant act on the lung fibroblast at different time points, the positive units of PLC of supernatant groups were higher than the control group and with the action time prolong, the positive unit of PLC increased, statistical significance was considered at P<0.05.When supernatant combined with OM to act on the lung fibroblast, the positive units of PLC were reduced in 30min and 60min,comparing with control group, statistical significance was considered at P<0.05. 4.The PKC results showed that: when supernatant act on the lung fibroblast at different time points, the positive units of PKC of supernatant groups were higher than the control group and with the action time prolong, the positive unit of PKC increased, statistical significance was considered at P<0.05.When supernatant combing OM to act on the lung fibroblast, the positive units of PKC of supernatant groups were higher than the control group, statistical significance was considered at P<0.05.When supernatant combined with OM to act on the lung fibroblast, the positive units of PKC were reduced in 30min and 60min,comparing with control group, statistical significance was considered at P<0.05. 5. Ca2+ results showed that: when supernatant act on the lung fibroblast at 30 and 60 min, the F value of supernatant groups were higher than the control group, statistical significance was considered at P<0.05.With the action time prolong, the F value increased, statistical significance was considered at P<0.05.When supernatant combing with OM to act on the lung fibroblast at 60min,the F value were reduced, comparing with supernatant groups, statistical significance was considered at P<0.05.Conclusion: 1. Quartz - macrophage culture supernatants can promote the proliferation of lung fibroblasts and the composition of collagen, there is a certain relationship between the effect and action time. 2. The expression of Gq protein of lung fibroblasts up-regulated and expand the effect during quartz - macrophage culture supernatant contributed to the process of fibroblast proliferation. 3. Quartz - macrophage culture supernatant promoting fibroblast proliferation may be through activation of Gq protein-mediated Ca-IP3 and DAG-PKC pathway. 4. OM may exert a biological effect by effecting Gq protein and interfering with Ca-IP3 and DAG-PKC signal pathway substances.
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