| BackgroundIschemia-reperfusion injury is a common organizations organ damage of general surgery practice. It play an important role in the evolution of the disease pathology such as infection, trauma, shock, cardiopulmonary dysfunction and so on[1-3]. The study of intestinal ischemia-reperfusion injury happened mechanism and look for its prevention and treatment measures have become a hot research in the field of surgery.ObjectiveThrough the establishment of rat intestinal ischemia- reperfusion injury model to observe the protective effect of tetramethylpyrazine on IRI and to expore its mechanism.Materials and MethodsUsing rat superior mesenteric artery occlusion method establish IRI model, 40 healthy wistar rats weighing 250±10g were divided into 4 groups which were sham operation group,ischemia-reperfusion group,Vitamin C group and tetramethylpyrazine group,respcectively.Sham-operated group: superior mesenteric artery open after the separation, not for blocking; Ischemia-reperfusion group:Separation superior mesenteric artery, using non-invasive arterial folder mesangial blocking its blood vessels 45 minutes after reperfusion; VitaminC group and Tetramethylpyrazine group: Wistar rats was injected vitaminC 180mg/kg and Tetramethylpyrazine 80mg/kg, by sublingual vein respectively at10 minutes before reperfusion. After 120 minutes of reperfusion, based sacrificed respectively, the abdominal aorta collected blood samples, check centrifugal plasma-700C save standby. And get away from the Director of 4 cm of small intestine caecum 5cm cut open, make detecting Intestinal histology and grading CHIU; The rest of the intestinal tissue were used for the production of pulp, -70℃to preserve the reserve. Separately with xanthine oxidase method, thiobarbituric acid method, enzyme-coupled UV spectrophotometry and synthetic chromogenic substrate Limulus Test Method Determination of plasma and intestinal tissue were plasma superoxide dismutase (SOD) vitality, malondialdehyde (MDA) levels and plasma D-lactate, endotoxin levels.Statistical calculations were done by SPSS13.0 for windows.Results 1. Pathological changes in intestinal tissue: Compared with the sham-operated group, IRI model group and Vitamin C group's intestinal mucosal of rats with severe damage (P <0.05), while there was no significant difference in the degree of intestinal mucosal injury between Tetramethylpyrazine group and sham-operated group (P> 0.05); Compared with the IRI model group,Vitamin C group and Tetramethylpyrazine group have different degree of reduction in intestinal mucosal injury of rats (P <0.05), we could see the Tetramethylpyrazine group was significantly slower than Vitamin C group(P <0.05).2. The experimental group measured changes in biochemical markers:1) The SOD level of serum and intestinal tissue: Tetramethylpyrazine group's SOD level of serum and intestinal tissue was significantly higher than IRI model group and Vitamin C group (P <0.05), while compared with the sham-operated group, it was no no statistic significance (P> 0.05).2) The MDA level of serum and intestinal tissue: Tetramethylpyrazine group's MDA level of serum and intestinal tissue was significantly lower than IRI model group and Vitamin C group (P <0.05); But obviously higher than that of sham-operated group (P <0.05). In addition, Tetramethylpyrazine group was significantly lower than Vitamin C group on MDA level of serum (P <0.05), but there was no statistic significance in MDA level of intestinal (P> 0.05).3) The D - lactic acid level of serum: Tetramethylpyrazine group's D - lactic acid level of serum was significantly lower than IRI model group and Vitamin C group (P <0.05), But obviously higher than that of sham-operated group (P <0.05).4) The Endotoxin level of serum: Tetramethylpyrazine group's endotoxin level of serum was significantly lower than IRI model group and Vitamin C group (P <0.05), while compared with the sham-operated group, it was no no statistic significance (P> 0.05).ConclusionTetramethylpyrazine are able to remove oxygen free radicals , to inhibit lipid peroxidation, to prevent endotoxin translocation, to improve the pathological changes and reduce intestinal injury grading.It play a protective a role for IRI and its effectiveness better than Vitamin C.
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