Effect Of Leptin Of Severe Acute Pancreatitis Associated With Lung Injury Rats.

Objective:To explore the relationship between expression of iNOS,NO,NF-κB activation of lung tissue and lung injury in rats of severe acute pancreatitis(SAP) associated with lung injury and to explore the effect of leptin.Methods:Thirty six Wistar rats were randomized into the three group:normal group(groupA);SAP group(groupB);leptin treatment group(groupC).The SAP model was induced by injection of 5%sodium taurocholate solution into biliopancreatic duct.All the animals were sacrificed in 6h.Activation of NF-κB was determined with immunohistoehemistry.Mean-while,iNOS,NO,plasma leve1 of amylase were determined.lavage was introduced in their lobes of right lung.The lavage fluid was counted leucocytes.In the end,lung and pancrea tissues histological were determined.Results: In SAP group, the NF-κB activity,the NO levels and the iNOS expression increased markedly.In Leptin group, the activation of NF-κB was prevented and plasma level of amylase , and content of NO and iNOS remarkably decreased.Activation of NF-κB had positive correlation with expression of iNOS (r=O.79,P<0.01) and iNOS had positive correlation with NO (r=0.66,P<0.01).At sixth hour,the arterial blood gas,serum amylase,NO and were not obviously different between groupA and groupC.The leucocytes counts were higher in groupB than those in groupsA andC.By microscopic observation of the histological appearance and the cells of lung and pancrea resulted in the diagnostie standard of the serve acute pancreatitis with lung injury in groupB.Conclusion: This is a successful method of making model of experimental serve acute pancreatitis associated with lung injury; NF-κB activation,iNOS expression and NO level were very important in inducing experimental acute pancreatitis associated with lung injury.Early blockage of NF-κB activation with leptin is effective for reducing severity of the lung injury.Leptin could reduce iNOS expression and NO level by inhibiting the NF-κB activation.