Study On The Anti-colorectal Cancer Effect And The Mechanism Of Dihydroartemisinin

Objective:To study on the anti-colorectal cancer effect of Dihydroartemisinin and further explore the mechanism of action.Methods:1.In order to understand the current situation of research on colorectal cancer and Dihydroartemisinin,we look up and analyze a large number of domestic and foreign pertinent literatures.2.The methods of inoculating nude mice with humancolorectal cancer LoVo which have been well cultivated are used to establish the model of transplanted tumor in nude mice.After that,we divide the nude mouse into five groups and each groups of ten.To intragastric administed Dihydroartemisinin according the low dose,middle dose and high dose(30mg/kg,60mg/kg,120mg/kg) for 14 days.The tumor suppression effects of Dihydroartemisinin were studied from the following aspects:the xenograft of human colorectal cancer were excised and weighted to calculate the tumor growth inhibitory rate.3.To prepare the pathological section of the xenograft of human colorectal cancer tissue with the routine method,and then use the method of SABC immunohistochemical methods to detect the expression of proteinic factor bcl-2 and VEGF in tumor-bearing nude mice.Result:1.The tumor growth inhibitory rate of the Dihydroartemisinin high dose is 36.54%, the tumor growth inhibitory rate of the Dihydroartemisinin middle dose is 26.55%,the tumor growth inhibitory rate of the Dihydroartemisinin low dose is 12.43%.This inhibitory action shows the dose-effect dependent.The average tumor weight of the high dose group which compared with the control group in difference has statistical significance(P<0.05). 2.The result of the expression of proteinic factor bcl-2 shows that the control group's positive rate is 69.62%,the group of 5-Fu is 56.15%and the group of the Dihydroartemisinin high dose is 63.66%.The Dihydroartemisinin high dose group which compared with the control group in difference has statistical significance(P<0.05).But The Dihydroartemisinin high dose group which compared with the control group in difference has no statistical significance in the aspect of the expression of proteinic factor VEGF.Conclusion:Dihydroartemisinin shows its anti-colorectal cancer effect by influencing the expression of the anti-apoptosis factor bcl-2.