| Background Cerebral vascular disease is regarded as one of the three major causes of death.Separately,the incidence and morbidity rate are 100-300/100 thousands(about 70%is CVD) and 500-740/100 thousands.And the death rate is 50-100/100 thousands(which is about 10%).The sequela,such as hemiplegia, anepia,dementia,etc.seriously affected the quality of life of patients and give much burden to them.Nerve cell necrosis and the reperfusion injury result in the neurologic impairment and hemiplegia after cerebral ischemia.The pathomechanism,which refers to the nerve cell injury,nerve cell denaturation and procedure apoptosis in ACI patients,is related with inflammation and immunological reaction after cerebral ischemia.In recent years,some researches show that interleukin-6 and tumor necrosis factor-alpha can effect on the inflammation reaction,which is enabled by ACI.Besides,the inflammation reaction is also regulated by suppressors of cytokine signaling-3. Large number of studies have shown that the Huatan Tongluo Decoction is an effective way to treat the ACI.However,the mechanism is still unknown. Nowadays,the researchers pay less attention to it,especially the relationship between socs-3 and inflammation reaction,which need further study to enrich the theory of traditional Chinese medicine. Objective To observe the effect of Huatan Tongluo Decoction on the expression of SOCS-3 in ACI patience with Phlegm wind resistante type.Methods①66 cases of ACI patients,admitted to the Foshan Hospital of TCM from June 2008 to February 2009,confirmed by CT or MRI,were randomly divided into treatment group(34 cases)and control group(32 cases);②According to the standard,the therapeutic effect was evaluated after 21-day treatment;③And the neurology grade in two groups were separately evaluated with the method of NIH Stroke scale and Barthel index;④Before treatment and after 7-day,the expression of SOCS-3 in two groups were respectively detect by ELISA and the expressiOn of IL-6 and TNF-αin two groups were respectively detect by chemiluminescence;⑤To analyze the correlation between expression of SOCS-3,IL-6 and TNF-αwith CIV.Results①There were significantly difference on excellence rate and total effective rate between two group(P＜0.05);②NIH Stroke scale in treatment group was lower than control group on the 14th day after treatment(P＜0.05); The advance of neurology grades,which was evaluated with the method of Barthel index,have marked difference(P＜0.05)between two groups on the 14th day and 21day after treatment;③The expression of SOCS-3,INF-αand IL-6 in ACI patients significantly increased than healthy people(P＜0.05).The expression of SOCS-3 in treatment group markedly increased than its own prior treatment on the 7th day,being significantly different(P＜0.05).However,the expression of TNF-αafter post-treatment was decreased than its own prior treatment on the 7th day,being significantly different(P＜0.05);④There were no significantly difference between CIV and the expression of SOCS-3 (P＞0.05).However,there was positive correlation with the expression of IL-6 and TNF-αand CIV(γ=0.927,P＜0.05,γ=0.757,P＜0.05);⑤Barthel grade has the positive correlation with the expression of SOCS-3 in prior treatment (γ=0.810,P＜0.05)but has the negative correlation with the expression of IL-6 and TNF-α(γ=-0.672,P＜0.05,γ=-0.822,P＜0.05) Conclusions①The SOCS-3,IL-6 and TNF-αinvolves in the regulation process of inflammatory reaction after ACI diseases.②Huatan Tongluo Decoction is an effective way to treat the ACI,which can significantly improve neural functional grade.③The mechanism may be closed related with the up regulation to the expression of SOCS-3 down regulation to the expression of IL- 6 and TNF-αand Huatan Tongluo Decoction can relieve inflammatory reaction after cerebral ischemia,from the aspect of the up regulation to the expression of SOCS-3 and down regulation to the expression of IL-6 and TNF-α,which is to be as a latest vital mechanism. |
PDF Full Text Request