Studies On Defibrillation Effect And Side Effects Of Biphasic Wave Defibrillator

Objectice: To study defibrillation effect and side effects of the biphasic wave defibrillator.Methods:1. Six Taihu-pigs, for estabilshing ventricular fibrillation (VF) model, electrode was induced to the right ventricular. Defibrillation waveform used MDBTE, GEMDS and PCBTE as contrast, asynchronous defibrillation in vitro, obtained separately the defibrillation energy(E50) and peak current(I50) of the waveform impedance groups.2. Three Beagle dogs, defibrillation waveform used MDBTE, sequence setting for waveform energy was 70J, 100J, 200J and 360J, for defibrillation success rate of waveform synchronizing with the R-wave.3. Six Taihu-pigs, defibrillation waveform used MDBTE, GEMDS and PCBTE as contrast, asynchronous defibrillation in vivo the defibrillation energy were 5J, 10J and 20J, calculate the defibrillation success rate of the waveform-energy group.4. Thirteen Taihu-pigs were randomly divided into MDBTE group and PCBTE group, each group had 5 pigs, the defibrillation energy for two groups of waveforms were 360J, and the defibrillation shock of each Taihu-pigs was 10 times. 72h after defibrillation, Romving Taihu-pig's heart to do the pathological examination and electron microscope examination,romve the skin under the electrode to do the pathological examination, and samples venous blood to determine cardiac troponin (cTnT).Control group had 3 pigs, only for anesthesia, no defibrillation, 72h after defibrillation, romving Taihu-pig's heart and the skin under the electrode to do the pathological examination and electron microscope examination, as the control group of side-effect assessment.Results:1. In the measured impedance case, E50 and I50 of MDBTE was obviously smaller than results of GEMDS (P<0.05), and there are no differences between MDBTE and PCBTE (P>0.05). 2. When defibrillation energy were 5J, 10J and 20J, MDBTE defibrillation success rates were 55.3%,95.7% and 97.9%, the control group GEMDS defibrillation success rates were 19.1%,68.1% and 83.0%,and the control group PCBTE 59.6%,93.6% and 100%. 3. When discharge energy was 70J, 100J, 200J and 360J, the R-wave synchronization success rate of MDBTE were 100%. 4. After MDBTE and PCBTE defibrillation shock, the major pathological findings consideration was myocardial fiber breakage, of which there were three cases in MDBTE group, and two cases in PCBTE group. The skin of defibrillation district was epidermal integrity, and no bleeding and coagulation necrosis in collagen fibers. In MDBTE group, the muscle cell nucleus forms were normal and sarcomere neatly arranged;in PCBTE group, the muscle cell nucleus were integrity and the structure of the mitochondrial was normal.72h after defibrillation, the cTnT content of MDBTE group was 0.78±0.43ng/ml, and the cTnT content of PCBTE was 0.52±0.24ng/ml.Conclusions: In vivo and vitro defibrillation effect of MDBTE has a better efficacy and safety compared with GEMDS, and has a considerable effect with PCBTE. MDBTE and PCBTE have no significant differences in side effects.