The Study Of Estrogen Receptor In Adenomyosis And Endometriosis

Objective:To investigate the effects of selective estrogen receptor-βagonist (WAY-200070) on focus development of endometriosis(EM) and adenomyosis (AM),provide the data of treating endometriosis(EM) and adenomyosis(AM).Methods:Eutopic endometrium of endometriosis,ovarian endometriotic cysts,adenomyotic focus and normal endometrium were subcutaneously transplanted into SCID mice,and the SCID mice models were established.Then the next day the mices were randomized into treatment group received WAY-200070(5mg/kg·d) by subcutaneous injection and the control group received NS above.The treatment regimen lasted 14 days.Measure the weight of the lesion after killing the mices.HE and Immunohistochemistry and RT-PCR were used to determine the interstitial substance and gland and the expression of ERβand ERαin ectopic tissue.Results:Compared with the control group,the weight of lesions that were obtained from Eutopic endometrium of endometriosis,ovarian endometriotic cysts and normal endometrium were not reduced in mices treated with WAY-200070(P＞0. 05).Compared with the control group,the weight of lesions that were obtained from adenomyotic focus were reduced in mice treated with WAY-200070(P＜0.05). Expressions of ERβfrom these four groups were alike in the groups using drug and the control groups,and Expressions of ERαfrom the first three groups were alike in the groups using drug and the control groups.Otherwise,On light microscopy,the interstitial substance and gland of adenomyotic ectopic lesions were significantly different in the group using WAY-200070 than the control group(P＜0.05).At last, the macrophage and nature killer cells were tested in adenomyotic focus and they were all alike in the groups using drug and the control groups(P＞0.05).Conclusions:selective estrogen receptor-βagonist can not effectively interfere with the growth of endometiosis.In contrast,It may make some effects on the growth of adenomyosis,otherwise,It is an puzzle that how make these changes.It is obvious that the macrophage and nature killer cells make no use on these changes in this experiment.