The Expression And The Relationships Among TLR4, MBP, GFAP, And PDGFR-α In The Brain Of The Premature Rats With Intrauterine Infection

Objective The aim of the study is to explore the mechanisms of endotoxin induced white matter damage(WMD) after intrauterine infection of rats,so as to provide experimental evidences for clinical diagnosis and treatment to WMD of prematures.Methods Pregnant rats were randomly divided into two groups:infection group and control group.The infection group was established by intraperitoneal injection of endotoxin in pregnant rats when gestation was 70%complete(15 days).The control group was treated with normal saline.The fetal rats were killed after maternal endotoxin administration2,4,12,24and72hours respectively.The pathological changes in placenta and in fetal rat brain were determined by hematoxylin and eosin(HE) staining.Using fluorescence RT-PCR to examine the expression of TLR4 in fetal rat brains.Immunohistochemical method was used to detect the expression of GFAP,MBP and PDGFR-α.Results After maternal endotoxin administration,infiltration of neutrophil in the placenta,weak staining of cerebral white matter and focal rarefaction in the fetal rat brains were observed.Expression of TLR4 mRNA in the fetal brain was decreased in 2h and 4h after the administration of LPS.In the endotoxin-treated group,positive staining of MBP and PDGFR-αin the fetal rat brains was much less and weaker in 12h,24h,72h;the increased expression of GFAP was observed in 12h,24h,72h. Conclusions Endotoxin can be used to establish intrauterine infection models and the infection may cause the lesion of the white matter.TLR4 participate in the course of the injury introduced by LPS.