Astragalus Radioactive In Brachial Plexus Injury In Rats In The Study Of The Protective Effect

Cancer treatment radiation therapy are one of the important ways. Brachial plexus as the composition of peripheral nerve is a common complication of radiotherapy. With the improvement of tumor treatment, the patient survival was significantly prolonged, radiotherapy-induced brachial plexus injury caused by dysfunction cause for concern.Objective:The subject of radioactive arm through the establishment of rat model of nerve injury from the use of general and tissue morphology detection, electrophysiological and immunohistochemical techniques to study the radiation damage of peripheral nerve after spinal cord anterior horn motor neuron, peripheral nerve and muscle changes in disposable , astragalus treatment group compared with the control group, the extent of nerve injury to explore the application of astragaloside the feasibility of prevention and treatment of complications of radiotherapy.Methods:Split-dose irradiation used program, in the rat brachial plexus injury in animal models of radioactive Huangqi applications. Rats were randomly divided into the application of Astragalus injection of irradiated group (experimental group) and the simple exposure group (control group), after injury, according to the different subjects into injury time after 3 weeks, 7 weeks and 12 weeks and 36 weeks 4 hours group each time group and the control group 4 rats, each time for the exposure group to the right side, using the next day exposure to 2 Gy / 1 times the cumulative dose reached after 30 Gy, respectively, in the last three weeks after irradiation, 7 weeks, 12 weeks and 36 weeks after the subjects. Experimental group were determined with the control group of rats the diameter of the upper arm of the arm circumference, biceps muscle wet weight, exercise biceps compound action potential and the potential of the incubation period. HE staining, silver staining and toluidine blue staining biceps lesions, on both sides of the spinal cord anterior horn motor neurons and the number of morphological changes and changes in the axon. Immunohistochemical method to detect c-fos in the former motor neurons expression.Results3 weeks after irradiation, the experimental group of animals does not appear obvious signs of brachial plexus injury, only pre-emergence of cases to check hyperemia, edema, hemorrhage, nerve cell lesions without details. Control group of rats was beginning lame gait appeared Show claw reflex; 3,7,12,36 weeks after irradiation, the control group compared with the experimental group arm circumference and biceps wet weight were significantly decreased (P <0.01 or P <0.05), HE staining visible biceps atrophy. EMG latency was significantly prolonged, electromyographic action potential decreased, were significantly different (P <0.01 or P <0.05). At 3 weeks after irradiation, the longest incubation period EMG, electromyography action potential minimum, after a few weeks has improved. Brachial plexus injury, the experimental group, lateral irradiation the number of surviving neurons with time significantly reduced, there is significant difference (P <0.01), shows that neuronal shrinkage, nuclear dissolution and other signs of cell death. Irradiation finished to 3 weeks after irradiation, spinal cord anterior horn motor neurons to reduce the number of very fast, at 3 weeks after irradiation, spinal cord anterior horn motor neurons to reduce the number down. Musculocutaneous nerve axonal degeneration in part serious, there is some nerve fibers myelin disintegration spherical, oval, rod-like structures. Immunohistochemistry showed that 3 weeks after irradiation, spinal cord anterior horn motor neurons, there are obvious c-fos staining, then, c-fos positive cells decreased. 12 weeks after irradiation, c-fos-positive cells occasionally.Conclusions:Analog breast radiotherapy using cumulative doses of 30 Gy irradiation dose segmentation program, in the rat brachial plexus injury in an animal model of radiation on the application of basic astragaloside agents. The experimental results show that high-dose X-ray radiation which may render the use of astragalus preparations did not appear brachial plexus injury in rats, exposure to side-gait and reflex abnormalities Show claw, arm biceps atrophy, abnormal EMG; high-dose X-ray radiation exposure will enable the lateral spinal cord anterior horn motor neuron survival decreased significantly the number of neuron cells lesions, axonal degeneration and myelin degeneration of nerve fibers; At the same time, and spinal cord neuronal apoptosis-related gene c-fos-positive expression. Irradiated group and astragalus does not appear obvious signs of brachial plexus injury, case check only hyperemia, edema, hemorrhage, nerve cells no significant lesions.