| Ku protein is a DNA-binding protein, with DNA and DNA damage lead tothe end of the double-stranded DNA binding fracture. Ku protein, Ku70 and Ku80from the two-subunit heterodimer constitutes the first by Japanese scientists in1981 first in the serum of patients with connective tissue in the form ofautoantibodies was found. Ku protein, the existence of a wide range of differentbiological tissues. At the same time and found it in the cell survival, growth in avariety of biological processes play a role, for example, DNA repair, themaintenance of telomere stability and regulation of apoptosis, resulting in tumordrug resistance and so on, is the view that the Ku protein in maintaining genomicstability, to strengthen the stability of cells and organisms play a key role. Kuexpression in normal tissues are important, Ku overexpression can lead toexcessive cell proliferation and apoptosis resistance; Ku less than the expressionof genes can lead to instability and tumorigenesis. Apoptosis and cancer cells arederived from DNA damage, DNA double-strand break DNA damage is DSBsrepair one serious injury, such as the timely repair of cells is fatal. Mammaliancells repair DNA DSBS homologous end of the reorganization of the way HR,non-homologous end-repaired NHEJ, but in some organizations, such as epithelialcells of bladder cancer compared with micro-homologous end joining MMEJ.Ku70 as a widely expressed DNA repair protein expression in the liver has acertain significance. Body to the liver as the main metabolic function of an organ,to play an oxidation, glycogen storage, secreted protein synthesis and other functions. Hepatic secretion of bile, the digestive gland the body's important. Theliver is an important metabolic organ. Liver in vivo and in vitro from a number ofnon-nutritive substances such as drugs, poisons, and certain metabolites in vivo,with the role of biotransformation. Liver disease is common in China, as the greatpowers of hepatitis, hepatitis B and hepatitis C in China reached 160 millionpatients, 3.2% infection rate. Hepatitis and cirrhosis caused by the large number ofpatients with primary liver cancer, liver cancer mortality, cancer mortality in thesecond sequence in the stomach, esophagus third door; in some rural areasaccounted for the second place, second only to gastric cancer. Annual incidence ofliver cancer around the world about 626,000 people, the death of 598,000 people.China's annual incidence of about 34 million people, about 30 million people died.Intrahepatic bile duct stones has also become common. Important physiologicalfunctions of the liver can not be separated from the basic unit of the liverhepatocytes, by studying the various types of liver cells in the Ku70 expression inan attempt to understand the various types of liver cells in functional status,disease prognosis and guide the follow-up treatment have important practicalvalue. Liver tissue, especially the expression of Ku70, and for liver cancerradiotherapy and chemotherapy after resection of guiding significance. The role ofthe general principles of chemotherapy is damage chromosomes, resulting in DNAfragmentation, the termination of cell growth, and whether it is in normal cells ortumor cells to express a wide range of Ku70 in DNA DSB repair, the process ofapoptosis regulation plays an important role. Ku70 research on the reversal oftumor resistance is important.The immunohistochemical method (SP method) was used to detect Ku70 in50 cases of liver cancer, 6 cases of cirrhotic tissue adjacent to cancer, 11 cases ofhepatolithiasis 1-induced liver inflammation and injury exception to the normalliver resected tissue to breast cancer Organization for the positive control,preliminary study of different liver in the human Ku70 Expression andsignificance. Ku70 in 50 cases of liver cancer cells, there are 35 cases of varying degrees of expression, no expression that is negative in 15 cases, in liver cirrhosisand liver inflammation and normal liver cell cytoplasm, were positive. Ku70expression in hepatocellular carcinoma and adjacent liver inflammation and livercirrhosis tissues difference statistically.Combination of experimental results, we draw the relevant conclusions:1.Ku70 as cell DNA-binding protein in the majority of hepatocellular carcinomaand liver tissue inflammation, cirrhosis of the liver and adjacent normal liver cellsin the expression of varying degrees. Ku70 that generally in these cells play afunctional DNA damage repair. 2.Ku70 in a small number of liver cancer cells forthe negative, because Ku70 as an important factor in DNA repair, Ku70-negativepatients with hepatocellular carcinoma after DNA damage repair capability, thedevelopment of liver cancer a key role in objective assessment condition. Such asstrengthening the expression of Ku70, so that patients with such liver's ability torepair DNA damage recovery, is bound to follow-up treatment of patients withsuch a valuable reference. At the same time, the negative Ku70 expression of thepathological diagnosis of liver cancer has a certain significance. 3. Ku70 in thethree cases of liver cancer cells for strong positive, postoperative radiotherapy andchemotherapy may be the existence of a certain degree of resistance, such asthrough SiRNA reduced Ku70 expression, for to achieve a satisfactory effect is acertain degree of clinical significance..
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