Expression Of FOXC2 On Gestational Trophoblastic Neoplasia

Gestational trophoblastic disease is one of the most harmful diseases which threaten women's health and their life. At present, benign diseases in GTD show a tendency of rising, while malignant Gestational trophoblastic diseases show downward trend, Nevertheless, patients afflicted with GTD can't breed a baby frequently. Therefore, the research about the occurrence,developing and observation on the clinical therapeutic efficacy of GTD is very important. Gestational trophoblastic disease is a group disease originate from placenta villus trophoblast.Embryo trophoblast has the ability to plant into the innermembrane of uterus. It is similar to the invasion of malignant tumor. Experiment on animals indicate, there is significant effect of Foxc2 transcription factor on genesis and development of mice embryo. Because Human FOXC2 proteins is more than 94% homology with mouse Foxc2, so we can speculate this gene should also play an essential regulatory role in human embryo development.Experiments apply the means of Immunohistochemistry staining have already confirmed this speculation: FOXC2 transcription factor have a wide distribution in early embryo of human.In gynecologic malignancies, including cervical cancer, endometrial cancer and ovarian cancer, the expression of FOXC2 have discovered, meanwhile, FOXC2 play a key role in the ivasion and metastasis of tumors.Objective: Investigate the expression of FOXC2 in gestational trophoblastic disease, in the hope of revealing the relationship between the FOXC2 transcription factor and the occurrence and developing of GTD. To seek a new examination index which can discover whether the hydatidiform mode will transform to malignant disease earlier.Also to find a new treatment methods.Methods: Using the means of immunohistochemistry staining and hematoxyl- ineosin staining to detect the expression of FOXC2 in gestational trophoblastic disease, including ten cases of normal villimole, ten cases of normal placenta, ten cases of hydatidiform mole (HM), nine cases of invasive mole(IM), five cases of choriocarcinoma (CC), and one case of placental site trophoblastic tumor.Results: The experimental results demonstrate that FOXC2 protein is mainly located in endochylema, a small number of cell membranes and cell mesenchyma are colored, the rate of positive expression of FOXC2 in normal villimole, normal placenta, hydatidiform mole,invasive hydatidiform mole, choriocarcinoma is 20%, 10%, 30%, 77.8% and 100% respectively. It also shows expression in one case of placental site trophoblastic tumor. The number of the cell staining positive and the strength of the staining increased in the wake of the addition of trophoblastic cell grade malignancy. All the tissues are different from each other in statistics (p<0.01). The expression of FOXC2 is higher in Gestational trophoblastic diseases than normal villimole and normal placenta (0.010.05). The expression of FOXC2 is obviously higher in invasive hydatidiform mole, choriocarcinoma than in hydatidiform mole,they are different from each other in statistics (0.01