| Myocardial infarction are the common diseases against human health and have high mortality rate, bring enormous pressure to society and family in recent years. a number of new drugs has been studied for treating and improving the prognosis of myocardial infarction ,especially for EPO research. many scholars at home and abroad, through animal experiments ,have proved that EPO have significant protective effect and unique advantage for myocardial infarction. This experiment to observe the protective effect of EPO on ischemic myocardial,through ligating the anterior descending coronary artery to establish acute myocardial infarction model in rats.In the experiment, we have chosen 32 healthy male and female Wistar rats of clean grade as research subjects ,and they were randomly divided into 4 groups of 8, into sham operation group, pre-treatment group, treatment group, the model group. Rats were weighed before the experiment, 30 minutes before coronary artery ligation, rats of pretreatment were given intraperitoneal injection of rhuEPO (5000U/kg), the remaining rats were given intraperitoneal injection of saline (5000U/kg). Ether inhalation anesthesia, Yang-bit fixed, use the multi-channel ECG monitor (model 90369, United States Spacelabs Medical) for ECG monitoring, record standard ECGâ…¡. Along the left sternal edge of the first intercostal thoracotomy 4, cut the pericardium, exposing the heart, at the lower edge of the left atrial appendage Department about 2mm left anterior descending coronary artery below the threading, with the exception of sham-operated group not only wears Line artery ligation, and the rest of the group were ligated LAD. To observe the electro electrocardiogram changes after ligation. In addition to the sham-operated group, the remaining rats in each group Hsintien ST-have shown high, T-wave high-sharp performance, it proved successful model. After ligation 30 minutes ,treatment group were given rhuEPO intraperitoneal injection (5000U/kg). The rest of the group at 30 minutes after ligation were given dose intraperitoneal injection of normal saline.24 hours later, remove one of the eyeball ,moving blood 2ml, to determine serum enzyme after centrifugation . 72-hours later once again to the determine the enzyme.Then all rats were put to death, check parts of each rat apical myocardium,used for electron microscopy, NBT staining for infarc size measurementt, RT-PCR technology to detect EPOR mRNA expression level in the gene.Experimental results show that: 1,RT-PCR showed that compared with the sham group, EPOmRNA expression level of pretreatment group,treatment group and model group are higher . the level of EPOR expression in the gene of model group is significantly higher than the other three groups but no statistical significance; 2,The serum enzymes (CKMB, LDH, AST) of rats in EPO pre-treatment group and treatment group with acute myocardial infarction was significantly lower than that in the model group 3,Myocardial infarct size of EPO pretreatment group and treatment group is significantly smalller than the model group, P <0.05, that is statistical significance.4,Electron microscopy:â‘ the sham operation group: normal myocardial ultrastructure. Neatly arranged myofibrils. Each with clear sarcomere. Neatly arranged mitochondria, the size of the normal form, capsule integrity, ridge-intensive rules; myocardial nuclei were spindle-shaped, rich in euchromatin, distribution, less heterochromatin. Obvious nucleolus.â‘¡treatment group: oval-shaped nucleus, large nucleolus, dense cytoplasm mild, with sarcomere difficult to distinguish between light and shade, occasional mitochondrial swelling, vacuolar degeneration, an increase in liposomes; occasional focal myofilament dissolution, glycogen decreased significantly.â‘¢pretreatment group: tow muscle, the sarcomere structure clearly visible separation of intercalated disc, partial mitochondrial compensatory hyperplasia, proliferation of small amount of lipid droplets, occasional sarcomere contraction.â‘£model group: myocardial ultrastructure apparent damage, mitochondrial disorder with obvious swelling and the breakdown of part of envelope, cristae broken, dissolved, some vacuolar degeneration of myocardial cells tend to shrinkage, decreased euchromatin, heterochromatin obvious increase, some a high degree of contraction-like sarcomere, actin filament dissolution.Conclusion: 1,acute myocardial infarction increased the expression of EPORmRNA. 2,The serum enzymes (CKMB, LDH, AST) of rats in EPO pre-treatment group and treatment group with acute myocardial infarction was significantly lower than that in the model group 3, myocardial infarct size of rats in EPO pre-treatment and treatment was significantly lower than the model group.s. 4, the pathological changes of myocardial ultrastructure of rats in EPO pretreatment group and treatment group are better than the model group.5,EPO and its receptor have myocardial protection on experimental rats with acute myocardial infarction ,... |
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