The Protective Effect Of Exogenous Zinc Sulfate On The Liver Of Diabetic Rats

ObjectiveObjective: To observe the effect of exogenous zinc sulfate on STZ- induced diabeticra tmodel of liver injury in rats and its mecha nism.Methods: 50 healthy wistar rats were randomly divided into A, B, C, D, E into fivegroups. A group of norma l control group, B group of high-fat control group, C group of STZinduceddiabetic high-fat group (referred to as non-diabetic intervention group), D group ofSTZ-induced diabetic high-fat intervention group zinc (zinc group referred to as diabetes), Egroup STZ-induced diabetic fat intervention group after the zinc (zinc group after referred toas diabetes). D and E group to the concentration of zinc ions(Zn2+)15mg·Kg-1·d-1, givenZnSO4 solution(1ml·200g BW－1)administered daily, A, B, C in pure water gavage group .Model of diabetes for 8 weeks after the blood extraction eye, liver lipids test the blood sugar,the anima ls were killed by broken the neck, the liver index measurements of liver organ,made from some 10% of liver tissue measuring superoxide dismutase (SOD),ma lond ia ldehyde (MDA), glutathione peroxidase (GSH-Px), measured by immunohistochemistryof meta llothionein, HE staining.Results: Compared with diabetes compared to non-intervention group, zinc interventiongroup (D, E) in serum AST, ALT levels are on a downward trend, but no significa ntdifference (P> 0.05), D group and E group no significa nt differences (P> 0.05). Liver tissueMDA: diabetes mellitus non-intervention group and the zinc group and other groups weresignifica ntly different (P <0.05), diabetes mellitus non-intervention group and plus zincgroup tha n other groups of high value. Normal control group, high-fat control group anddiabetes group after the zinc was no significa nt difference (P>0.05), diabetes mellitus noninterventiongroup and the zinc group had no significa nt difference (P> 0.05). Liver tissueSOD: the intervention group with diabetes not the other groups were significa ntly different(P<0.05), diabetic group did not interfere with values lower tha n other groups. Diabetes pluszinc group and other groups were significa ntly different (P <0.05), SOD values tha n the high value of other groups, norma l control group, high-fat control group and diabetes group afterthe zinc was no significa nt difference (P> 0.05). Liver GSH-Px: Diabetes does not interferewith group and other groups were significa ntly different (P<0.05), values lower tha n othergroups, norma l control group, high-fat control group, diabetes mellitus plus zinc group andafter zinc group had no significa nt difference (P> 0.05). HE staining: non-intervention groupof diabetic fatty liver degeneration and necros is clear that diabetes and diabetes plus zincgroup after the zinc group tha n fatty degeneration and necrosis in the intervention group didnot reduce diabetes. Metallothionein immunohist- ochemica l determination of : diabetesmellitus plus zinc group and after the zinc group and the norma l control group, high-fatcontrol group, diabetes group did not interfere with a significa nt difference, diabetes anddiabetes plus zinc group after group of zinc meta llothionein expression more.Conclusion:Exogenous zinc sulfate can improve the diabetic rats not only by thepathology of liver injury, but also reduce the lipid metabolism disorders, liver dama ge andperoxide status. Its mecha nism of zinc sulfate ma y be related to the promotion of livermeta llothionein expression, thereby strengthening the role of anti-oxidation.