Research On A Novel Bioadhesive Made Of Marine Mussel Adhesive Protein

The characteristics of marine mussel adhesive proteins,such as strong intensity, tenacity,moisture-resistance and strong adhesive ability,are due to their peculiarity of molecular structure,cross-linking of bonds and the interaction between proteins and substrata mediated by DOPA.Furthermore,they are biocompatible and biodegradable. Therefore,marine mussel adhesive proteins may be a preponderant and potential bioadhesive.This thesis focused on the research of two new type of mussel bioadhesive,naturally extracted Perna viridis foot protein(Pvfp) and recombinant functional peptide of Mytilus coruscus foot protein 1(Mcfp-1).We extracted Pvfp from the feet of P.viridis according to acetone precipitation.At the same time, recombinant DNA technology was used to obtain the peptide of Mcfp-1 and named Mcfp-1-12.We analyzed the function of adhesive proteins obtained by these two methods.Results as follows:(1) The coating ability on several surfaces,such as glass slide,polystyrene cell culture plate,titanium sheet and aluminium plate were observed,and the results showed that coating ability of Pvfp and Mcfp-1-12 were stronger than CELL-TAK^TM.(2) Quartz crystal microbalance(QCM) showed that the adsorption ability of the two obtained adhesives were more than CELL-TAK^TM on gold surface.(3) Pvfp and Mcfp-1-12 could achieve cell adhesion within 1 h.Compared with CELL-TAK^TM,Mcfp-1-12 had similar ability and Pvfp behaved much better.In comparison with poly-L-Lysine(PLL),these two proteins had better adhesion ability and higher superiority in waterproof.(4) Pvfp and Mcfp-1-12 were biocompatible.(5) Under humid environment,plastic consumables could be adhered with very small quantity,as well as femur of mouse.In addition,the tensile strength of Pvfp was twifold higher than medical Fibrin adhesive in large scale test.These results suggested that mussel adhesive proteins made in this reseach had high-performance waterproof,strong adhesion ability and good biocompatibility.So they were expected to be a favorable bioadhesive in medicine.