Study Of NMR Analysis Technology Of Body Fluid In Metabonomics

The NMR-based metabonomics approach evolved from the pioneering work of Nicholson and co-workers in 1999 has become a novel analytical technique. NMR-based metabonomics closely related to NMR analysis technology. Effective water suppression, a good phase and the smooth baseline of the spectrum is essential to the follow-up statistical treatment in metabonomics. In this paper, NMR analysis technology in metabonomics is summed up, and NMR experiments with different sequences, shimming on different nucleus in metabonomic study were compared, and the use of absolute value of spectrum and manually and automatically phasing of the phase-sensitive spectrum in metabonomic study were also compared. The results showed that:1. ES-CPMG sequence is relatively better in stability, repeatability and more suitable for NMR-based metabonomic study of serum samples.2. In NMR-based metabonomic study of urine, the experiments without deuterium reagents, using ~1H nuclear shimming is the initial feasible.3. In the case of inconveniently phasing, the use of the absolute value spectrum data for metabonomic studies may be better and more accurate. By comparison of manually and automatically phasing of the spectrum, we have seen, automatic phasing program also need further improvement.