Modification On The Interaction Of Drug With Serum Albumin By Molecular Spectroscopy

In this thesis, molecule spectroscopy, such as fluorescence spectroscopy and UV-Vis spectroscopy, were used to study the interaction of drug and Serum Albumin. And, the modified fluorescence spectroscopy and the modified synchronous fluorescence spectroscopy were proposed and studied on the interaction between drug with Serum Albumin in the thesis. The scientificity and reasonability of the two new methods have also been verified.And, the interaction model of drug and Serum Albumin was disccused. There are four chapters.In the first chapter, the interaction between the phthalate esters with Bovine Serum Albumin were studied by classic fluorescence spectroscopy. The binding parameters of the interaction of the four of the phthalate esters and Bovine Serum Albumin have been calculated. The results show that the hydrophobic force is the main binding force of the four interaction systems. And, with the increasing of the hydrophobicity of phthalate esters, the binding parameter values of the interaction of the four interaction systems increase gradually. The results also show that with the increasing of the binding parameters value of the interaction of the phthalate esters and Bovine Serum Albumin, the acute toxicity of phthalate esters increases gradually. Based on the binding parameters and the hydrophobicity of Di-n-octy phthalate, the predicted result is that the acute toxicity of Di-n-octy phthalate is the maximum in the four phthalate esters.In the second chapter, based on the research approach of the study of the interaction between drug with protein by fluorescence method and aiming at overcoming the insufficiency of classic fluorescence spectroscopy, the modified fluorescence spectroscopy is proposed. The interaction of Ephedrine and Florfenicol with Bovine Serum Albumin were studied by the classic fluorescence spectroscopy, the modified fluorescence spectroscopy and UV-Vis spectroscopy respecitively in the chapter. The results show that the modified method could reflect the whole and true information of some interaction. The scientificity and reasonability of the modified fluorescence spectroscopy has also been verified by UV-Vis spectroscopy.In the third chapter, the interaction of Trihexyphenidyl, Primaquine, Sodium Dodecyl Benzene Sulfonate and citalopram with Human Serum Albumin were studied by the classic fluorescence spectroscopy, the modified fluorescence spectroscopy and UV-Vis spectroscopy respecitively in the chapter. The results also show that the modified method could reflect the whole and true information of some interaction. The scientificity and reasonability of the modified fluorescence spectroscopy and the fluorescence background subtracting method have also been verified by UV-Vis spectroscopy. That the interaction mode between drugs with HSA included to point-to-area has been drawn.In the fourth chapter, aiming at the problem of the interaction system with the overlap fluorescence spectra which can not be processed by static fluorescence quenching theory, the modified synchronous fluorescence spectroscopy was proposed. The interaction of Citalopram and Sibutramine Hydrchloride with Bovine Serum Albumin were studied by the classic fluorescence spectroscopy, the modified fluorescence spectroscopy, the modified synchronous fluorescence spectroscopy and UV-Vis spectroscopy respecitively in the chapter. The original spectra of the interaction of Citalopram and Sibutramine Hydrchloride with Bovine Serum Albumin, which can be processed by static fluorescence quenching theory directly, could be obtained by the modified synchronous fluorescence spectroscopy. The scientificity and reasonability of the new method has also been verified. The results show that the new method is much more suitable for the similar above interaction system. And, the fluorescence background subtracting method could introduce error, when the concentration of quencher (such as BSA) is much less than the the concentration of drug.