| Background and ObjectivesScleroderma is an autoimmunity connective tissue disease, its etiology and pathogenesis is unclear, it is characterized by excessive and widespread fibrosis in the skin and internal organs, vasculopathy and abnormal activation of the immune system. And fibrosis is the most remarkable clinic character in the process of scleroderma, it is also the major threaten of influencing seriously on the life quality of patients, even the patients'life. It was indicated by research that UVA could penetrate through the epiderm, dermis, even deeply to subcutaneous and had an influence on the tissue, cell, blood vessel and DNA of the local irradiated site to produce the function of adjusting the organism state and treating the disease. It had been verified that UVA had the function of anti-inflammatory, immunomodulation and improve the local microcirculation, and which had been applied on the treatment of other diseases of dermatology for many years. Recently, it was indicated by several researches that satisfactory curative effect had been gotten on the treatment of scleroderma with the UVA, but the attention about the mechanism of UVA therapying scleroderma have been paid very less.For the first time, on the basis of constructing BLM seleroderma mouse model, by observing the change of the appearance, histopathology and the contents of hydroxyproline and collagen of the local injected skin of scleroderma mouse to verify whether UVA can improve the sclerised skin of scleroderma mouse effectively, and determineing the change of cytokine-TGFβ1,TGFβ2 and decorin, which have the correlation on the pathogenesis and skin fibrosis of scleroderma, This research tried to explore the possible mechanism of UVA having the influence on scleroderma mouse model so that to provide the important reference evidence for understanding further the pathogenesis and exploring the therapeutic methods of scleroderma.Materials and MethodsThe scleroderma mouse model has been constructed by BLM of 300μg/ mL injecting the back skin of Balb/c mouse per day, all 28 days successively, then verified whether the scleroderma mouse model can been constructed successfully or not by observing the change of the skin appearance, histopathology of skin lesion and lung tissue, the contents of hydroxyproline and collagen of the local injected skin of scleroderma mouse. Then irradiate scleroderma model mouse induced by BLM with UVA1 which wave length is 365nm, observe the change of local hard skin,skin pathology,the content of hydroxyproline and collagen at pre-irradiation and postirradiation respectively, and determine the change of TGFβ1,TGFβ2 and decorin of every group with the method of immunohistochemistry and RT-PCR.Results1. It has been verified that the construction of scleroderma mouse is successful from the change of skin appearance, the pathology display of skin lesion and lung tissue, and the contents of hydroxyproline and collagen.2. At the pre-irradiation and postirradiation, comparing the irradiation group mouse with the model group mouse, we found that the scleris skin of the irradiation group mouse was softened, the subcutaneou adhesions of which is lessened apparently, the contents of hydroxyproline and collagen of which decreased remarkablely(P<0.05), and its pathology displayed that dermis stratum got thin, the alignment of collagen fiber puffed and its quantities reduced; it is displayed that UVA1 irradiation can improved the scleris skin of scleroderma effectively from the three aspects of the change of skin appearance, the pathology of skin lesion and lung tissue, and the contents of hydroxyproline and collagen.3. Through determining the change of TGFβ1,TGFβ2 and decorin at the pre-irradiation and postirradiation, it was displayed that TGFβ1,TGFβ2 was high expression, and decorin was low expression in the skin lesion of UVA1 pre-irradiation scleroderma mouse model, TGFβ1,TGFβ2 was down-regulation expression, and decorin was up-regulation expression in the skin lesion of UVA1 postirradiation scleroderma mouse model.Conclusions1. It was displayed that UVA1 phototherapy can improved the scleris skin of scleroderma mouse effectively, make it soft and recover its elasticity,make the contents of hydroxyproline and collagen decrease significantly, collagen fiber align curmbly and the quantaties of it reduce, not only from the change of skin appearance, but also from the pathology of skin lesion, and the contents of hydroxyproline and collagen. 2. We presumed that the mechanism of UVA1 taking influence on scleroderma mouse model may be related to down-regulate TGFβ1,TGFβ2 and up-regulate decorin through observing the change of TGFβ1,TGFβ2 and decorin at pre-irradiation and postirradiation.
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