| Objective To find the effect and mechanism of CKY to the ulcerative colitis rat model.Methods Totally 70 healthy Wistar rats were divided into 6 groups by random.Group A(10 rats):normal control group.Group B(12 rats):model control group.Group C(12 rats): CKY low dose group.Group D(12 rats):CKY moderate dose group.Group E(12 rats):CKY high dose group.Group F(12 rats):SASP control group group.The rats of Group A were made with 0.25mL of 0.9%normal saline enemal.The others groups were made with 100mg/kg TNBS plus 0.25mL mixing reagent.The second day after the rat model establishment,the administration by stomach of CKY as 11g/kg,22g/kg,44g/kg were performed to rats in Group C,D,E.The administration by stomach of CKY were performed as 11ml/kg once daily.The DAI standard score and morphology and pathohistology score of colon injury were performed after twenty one days.The activity of superoxide dismutase (SOD) and the expression of induced nitricoxide synthase(iNOS) in colon were determined.Results Extreme significant difference(P＜0.01)were found between Group E and B on the DAI standard score and morphology and pathohistology score of colon injury and the activity of SOD and the expression of iNOS in colon.Significant difference(P＜0.05) were found among Group D,F,B.Extreme significant difference(P＜0.01) were found between Group B,C and A.Significant difference(P＜0.05) were found between Group D,F and A.Conclusion CKY is an active drug to the ulcerative colitis rat model.The therapeutic effect in Group E is superior to that of Group F.The therapeutic mechanism of CKY may enhance the activity of SOD and decrease the expression of iNOS in colon.
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