| Cyanea nozakii is a kind of harmful jellyfish for marine ecosystem which bloom out in the Bohai Sea and Northern Yellow Sea of China. In fact, the jellyfish bloom and Harmful Algal Bloom are top two ecological disaster of Chinese marine ecosystem, threatening the fishery industry. In order to eliminate threaten of jellyfish bloom on the marine fishery industry of our country. It is desiderated to undertake the basic research about how to use it as a kind of new marine resource. From the environmental economic point of view, it is the best solution try to find the way to utilize it. About how to use it as a kind of resource, however, we know nothing. Nothing has been reported about the basic knowledge and information about basic components, as well as their function up to now.For the first time, the glycoconjugates from Cyanea nozakii have been investigated comprehensively, including if there is any glycoconjugate in Cyanea nozakii, if there is, what's the content, how to isolate and purify it, as well as if the glycoconjugate from Cyanea nozakii has any physiologic activity, or toxicity. The data and results collected and recorded on this thesis can be used as the basic information for the further research and development of Cyanea nozakii, as a kind of new marine glycoconjugate resources.The optimum extraction condition was as follows: Cyanea Nozakii was first treated by acid at 55℃for 4~8 h with the addition of 2.5 parts acetic acid per 100 parts of raw material, and then treated by trypsin for another 8 h at 45℃and pH = 6.8~7.0. After that, the mixture was centrifuged at 4℃, 8000 rpm for 30 min. The supernatant was then collected, dialyzed, and treated to remove free protein, including trypsin, and centrifuged again. The supernatant was then collected and added of 4 fold cold ethanol to precipitate the polymer. The precipitation was then collected, resolved in distilled water and freeze-dried.The yield was approximately 63.4% of total solid contained in raw material. And it was composed by 43% protein and 56% carbohydrate. SDS-PAGE showed that there were still a few bands that can be stained by Coomassie blue dye, but only the main one, with the molecular weight of about 30 kDa, can be stained by periodic acid-Schiff agent simultaneously, suggested that it is a glycoprotein. The GC analysis shown that ribose was the essential component in the product, which relative content was as high as 64.42%. Other monosaccharide identified were galactose, fucose, glycose, mannose, and arabinose, with the content of 11.61%, 9.96%, 8.60%, 2.83%, and 2.58%, respectively.The bioactivity of such product had been assessed via mouse. The result showed that it could prolong the coagulating time and bleeding time in vivo and lengthen the coagulating time in vitro. The APTT, PT, and TT of mouse could be prolonged significantly, and the APTT is longer than PT. It suggested that the product had the anticoagulation activity. The mechanism might be the co-action of the inhibition of intrinsic coagulation and the inhibition of extrinsic coagulation, and the inhibiting effect to intrinsic pathway is stronger than that to extrinsic pathway.
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