Essential Study On "the Lung Relating To The Skin And Hair, Opening Into The Nose."

Establishing a rat model due to pulmonary disease, to observe the pathological tissue changes of skin,hair and nasal mucosa,and related index changes,to study the essential theory of"the lung relating to the skin and hair,opening into the nose."The experiment choose 30 SPF Wistar rats,half male and half female,randomly divided into 3 groups,each 10:normal control group,lung qi deficiency group and wind-cold invading lung group due to lung deficiency.Through smoking,dripping lipopolysaccharicle(LPS) into trachea,and throwing wind-cold factors,establishing models of lung qi deficiency and wind-cold invading lung,observing the changes of skin,hair and nasal mucosa,collecting models of skin,hair and nasal mucosa,observing the pathological tissue changes. Detecting the changes of minum elements:Fe,Ca,Mg,Zn;detecting the expression of TGF-β1 in skin tissues through immunohistochemistry method,detecting the expression of EGFmRNA in skin tissues through RT-PCR;detecting the expression of Substance P in nasal mucosa through immunohistochemistry method;testing the content of IL-4,INF-γin serum through ELISER method.Using SPSS11.5 stat.software to analyze the Experimental datum.The experiment shows that skin and nasal mucosa tissue of lung qi deficiency group and wind-cold invading lung group take great changes:the content of Fe,Ca,Mg,Zn minum elements in hair reducing; TGF-β1 and EGFmRNA in skin tissues increasing;the expression of Substance P in nasal mucosa increasing;IL-4 in serum increasing,INF-γand INF-γ/IL-4 in serum decreasing ; wind-cold invading lung group changing more noticeable.The experiment proving the correlation of skin,hair and nasal mucosa in micro-aspect,revealing the essence of the theory"the lung relating to the skin and hair,opening into the nose.";offering the thought,method and evidence to explore the modern study and application of this theory.