| It is well known to all that with the decline of ovary function, most women who are round perimenopausal period appear the menopause symptom of different degree by future reunion, that effect work and daily life. In order to improve life quality of middle age and old people life quality and alleviate menopause symptom, clinical up have already started to use hormone replacement therapy (HRT).The estrogen is endogenous active material that maintains women's normal physiology and mental state essentially .In the central nervous system , it not only effect neural circuit of generation ,influence generation, but also effect the neural circuit of cognition function influence study and memory. In order to study origin mechanism about neuroprotective effect of estrogen. This experiment spay rabbits as model of women who are in perimenopausal period..Then after HRT to observe express of nNOS and Synaptophysin in rabbit brain . All this research work will provide the gist of the study of nervous system disease, especially the study of the physiology of nervous pathology.Test one:The immune cell chemical method(SABC) is adopted to sdudy the morphology changing of nNOS-positive neurons in in PVN,SON,AHA and SCN of female rabbit hypothalamus under light microscope following ovariectomy and estrogen replenish. System of pattern analysis is undertaken to determine the pit(SON) and descends Qiu before the brain area(AHA) and sees to cross beside up pit(SCN) etc. pit nerve type inside the regiment an oxidize the nitrogen matches the Mao(nNOS) male nerve dollar of the appearance structure carry on prognosticate;Adopt inside the nNOS male nerve of the system measurement of the picture analysis and the Tu touch the plain immunity responds an outcome of average light density value. Result showed that:1.The false surgical operation set rabbit descends to distribute to have a great deal of nNOS inside Qiu brain, with SON, PVN, SCN, AHA expression stronger, the bow form pit only has a little amount nNOS male cell;Ovary after cut off, the obvious to build up of the express of the male nerve dollar of the each nerve pit nNOS, namely the amount increase dyeing to deepen;The estrogen after cure expression and ovary of the male nerve dollar of the each nerve pit nNOS cuts off a set to compare to obviously die down.2.Cut off an ovary set a rabbit to descend Qiu each nerve of brain a pit Tu to touch a plain immunity set to turn to dye to obviously die down than the false surgical operation set, dye to become shallow, the rabbit descend Qiu each nerve of brain a pit Tu to touch a plain expression to show Zhao to build up after the estrogen treatment.Test two make use of the organization chemistry of the SABC immunity method, carry on prognosticate to the appearance structure of the each nNOS inside the rabbit sea horse male nerve dollar under the optical microscope;Adopt the male nerve dollar of the sea horse nNOS of the system measurement of the picture analysis and Tu touches plain immunity to respond an outcome of average light density value.Express as a result:1 distribute abundant.Cut off after the ovary an each area nNOS male cell expression to die down, estrogen treatment after the obvious to build up of the express of nNOS male nerve dollar.2 cut off the sea horse outstanding plain expression obviously dies down than the false surgical operation set after the ovary, estrogen treatment after, expression compare take an ovary set to obviously strengthen. |
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