The Biological Characteristics Of Spontaneously Malignant Transformed Cell Lines From Human Embryonic Muscular Tissue

ObjectiveAneuploidy is by far the most massive genetic abnormality of cancer cell, it defined as abnormal chromosome number. The hypothesis that aneuploidy may be the cause of cancer was presented one hundred years ago, nowadays, more and more studies have identified the possiblity of hypothesis. Whether the aneuploidy was an independent factor for carcinogenesis, there is no conformation. In this experiment, muscular cells were fused by PEG to create aneuploidy cells. As PEG is not mutagenic chemical, so it can not induce gene mutation, aneuploidy cells by this method could make us to observe whether the aneuploidy can initiate tumor formation, and we could investigate the aneuploidy hypothesis by this method.Materials and MethodsHuman embryonic muscular cells were cultured, then cells were fused by 50%PEG, and observed whether aneuploidy cells could be produced;At the same time, human embryonic muscular cells were treated with MCA and colcemid, aneuploidy cells produced by this method were set as positive control; human embryonic muscular cells without intervention were set as negative control. Then aneuploidy cells were checked by chromosome analysis and flow cytometry . Then, we observed whether the aneuploidy cell could transform into tumor cell.ResultsFCM indicated that cells fused by 50% PEG. and cells treated with MCA (3-methylcholanthrene ) and colcemid were aneuploidy cells,chromosome analysis indicate the number is abnormal , most aneuploidy cells were hypoploid , the minorities were hyperploid .Aneuploidy cells fused by 50% PEG. could proliferate,and one cell line become immortal. No tumor formation was identified.ConclusionNormal muscular cells could be fused by polyethylene glycol and aneuploidy cells were produced,. No cell was malignant transformed after long-term culture. ObjectiveIn previous experiment, we had established immortalized cell line, named MS0812, MS0504, MS3, MS4 and MS5, which were spontaneously malignant transformed from human embryonic muscular tissue in vitro. In this study, chromosome character of these cell lines was studied.Materials and MethodsChromosome in MS0812, MS0504, MS3, MS4 and MS5 sarcoma cell lines are analyzed by conventional method, the number and structure of chromosome were documented. FCM was applied to study the aneuploidy cells.ResultsThe abnormal number and structure of chromosome was identified in nearly all of the sarcoma cell lines, the chromosome number was beyond 46, and ring, linear and dicentric chromosomes were also identified. FCM indicated that all of 5 cell lines were aneuploidy.ConclusionMS0812, MS0504, MS3, MS4 and MS5 cell lines were aneuploidy, and there were a wide range of abnormal in chromosome number and structure. ObjectiveTo observe the effects of matrine on proliferation and apoptosis of human mutated sarcoma cell line MS0812.MethodHuman sarcoma cell line MS0812 were treated with matrine at different concentration and time. The inhibitory rates was detected by MTT assay; Agarose gel electrophoresis of DNA, flow cytometry and TUNEL were utilized to observe and detect the apoptosis of MS0812 cells .ResultsMatrine exhibited inhibitory effect on the growth of sarcoma cell lines of MS0812. The cytotoxicity was observed in the MTT assay, DNA agarose gel electrophoresis showed typical ladders of apoptosis, TUNEL-positive cells were identified by light microscope. The cells showing an apoptotic pattern when analyzed by flow cytometry after matrine treatment.ConclusionMatrine have the effects of inducing apoptosis and inhibitory effects on MS0812 cell line in vitro.