| Objective:To investigate the effects of Shenfu injection,an intravenous drug made from traditional Chinese medicinal herbs,on spinal cord ischemic injury in rabbit models.Methods:24 male New Zealand white rabbits were anesthetized by halothane. Animals were randomly allocated to four groups:group group A(n=8) was received physiological saline and group C(n=8) was received intravenous Shenfu injection(10 ml·kg-1) at a constant rate(1.5ml/min) with in 30 min before aortic cross clamping; Group B was received Methyl-Prednisone injection(MP)(120 mg·kg-1) in 30 min after reperfusion respectively;Neurologic status was scored by the Jacob system at 1,4,8, 12,24 h and 48 h after the operation.The animals were sacrificed at 48h after reperfusion and the spinal cord(L2-7) was removed immediately for histopathologic study,HSP27mRNA examination by RT-PCR and apoptosis rate by Flows Cytometry(FCM).Results:All animals survived in the experiment.The neurologic scores at each observation time of group B and group C were significantly higher than those of group A(P＜0.05).No difference was found between group B and C.The numbers of apoptosis neurons at anterior spinal cord of group B and group C were less than those of group A at 48 h after reperfusion(P＜0.05).No difference was found between group B and C.The expression of HSP27 mRNA of group B and C were significantly higer than that of group A(P＜0.05).Conclusions:Shenfu injection has good effects on chronic spinal cord ischemica reperfusion injury rabbit models,which maybe related to its promotion of HSP27, reduce the neuro apoptosis and histological microenvironment,protect important organs.
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