| Baekgroud: Spinal cord ischemia reperfusion injury is an serious secondary spinal cord injury. Trauma, arteriosclerosis, vasal abnormality, inflammation, vasal infarct, decompression after the diseases that can bring spinal cord compression, lessening the blood stream of the spinal cord temporarily ,because of clamping the aorta during the operation of aorta, all of which can engender different extent spinal cord ischemia.The lately reports have show that incidence of spinal cord ischemia eperfusion injury is 3%～18% assumably. At the present time, although a series of attempts have been made to decrease the risk of this devastating complication, the incidence of paraplegia or neurological impairment after these kinds of injuries is still high.Objective: To investiage the effects honghua injection on forepart of spinal ischemia reperfusion injury and the underlying mechanisms, with the purpose of providing new idea and experimental testimony to clinical treatment.Methods: New Zealand white rabbits(n=63) were randomly allocated to 3 groups: Group A (with honghua injection treatment, n=30), Group B(with normal saline treatment, n=30) ,and Group C(with no treatment, n=3). Spinal ischemia reperfusion injury was made by cross-clamping infrarenal aortic for 35min according to Zivin's method. Group A injected 2.0ml/kg HI through vein before aortic cross-clamping and injected with the same dosage of HI again every day. Group B treated with the same dosage of NS as Group A. Group C received no treatment. The somatosensory evoked potentialis(SEP), the neurological status, the histopathological changes of the spinal cord tissue and the normal motor neurons were evaluated to investiage the effects HI on forepart of spinal ischemia reperfusion injury. At different time points after spinal ischemia reperfusion injury, the superoxide dismutase(SOD) activity and the malondialdehyde(MDA) production of the spinal cord tissue were measured with the Xanthine Oxidase (XO) method and thiobarbituric acid (TBA) assay, and they were compared with normal spinal cord tissue's. The positive expressions of Bal-2 protein and Bax protein of the neurocyte were studied immunohistochemically. Statistical.analysis was performed to investigate the mechanisms that HI alleviated the spinal ischemia reperfusion injury.Result:①The latencies of Group A were significantly shorter than that in Group B at all phase points (P＜0.01), and the amplitudes of Group A were significantly bigger than that in Group B(P＜0.01).②The average neurological function score in Group A was significantly higher at all phase points (P＜0.05);③In pathological examination, the range of vacuolation and the inflammatory reaction were more lightly than that in Group B, and the number of normal neurocyte was higher than that in Group B.④The level of MDA in Group A was significantly lower than that in Group B (P＜0.01),0 and the activity of SOD was significantly elevated in Group A than that in Group B (P＜0.01),⑤There was a weak positive expression of Bcl-2 protein at 4h after injury. By ld after injury, the positive expression rate of Bcl-2 protein was maximal. And the positive expression rate of Bcl-2 protein was higher in Group Athan that in Group B (P＜0.01).⑥There was a weak positive expression of Bax protein at 4h after injury. By ld after injury, the positive expression rate of Bcl-2 protein was maximal. And the positive expression rate of Bax protein was lower in Group A than that in Group B (P＜0.01).Conclusions:Honghua injection can significantly prevent and reduce neurologic injury induced by spinal cord ischemia reperfusion injury, the underlying mechanisms may be:①alleviating the lipid peroxidation of the spinal cord;②promoting the expression of Bcl-2 protein and restrain the expression of Bax protein in early spinal ischemia reperfusion injury, so as to restrain the apoptosis of neurocyte and alleviate the secondary spinal cord injury.MeSH Term: Spinal Cord Ischemia; Reperfusion Injury/TCD Therapy; Evoked Potentials, Somatosensory/drug effects; Neurobehavioral Manifestations/drug effects; Spinal cord/pathology...
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