Studies On Chemical Constituent And Antibacterial Activity Of Potentilla Kleiniana Wight Et Arn.

PotentiUa kleiniana Wight et Arn.(Rosaceae,Potentilla) is used whole herb as medicinal and has the efficacy of clearing heat,detoxification,dispelling wind and relieving cough,and can be used to malaria,cough,laryngalgia,erysipelas,tinea and hurts by snack or insect biting.Some researches had been undertaken for the antibacterial activity and chemical composition of Potentilla kleiniana Wight et Arn., the results as follows:The EtOH extract of Potentilla kleiniana Wight et Arn,was extracted by P.ether, CHCl₃,EtOAc and n-BuOH in turn,alter recovered solvent,four partial extracts were obtained.The antibacterial activities of each extraction were tested by the Escherichia coli,Staphylococcus aureus,Pseudomonas aeruginosa,Bacillussubtilis and Micrococcus luteus,and the results showed that the main active compounds distributed in the ethyl acetate extract.Then,the EtO Ac extract was isolated with the elution system(CHCl₃:EtO Ac),and 13 eluting components got.The antibacterial activity of each elute was tested by paper disk method.The inhibitory effect of the elutes of CHCl₃:EtOAc=1:0,50:1,20:1,10:1,8:1,5:1,3:1 are very obvious. Therefore,the antibacterial activity ingredients mainly exist in these elutes.Nine purified compounds were isolated from the EtOAc extract by using various kinds of chromatographic separation technology,and four compounds of them were identified by spectroscopic technique(GC-MS,¹H -NMR,¹³C-NMR) and with using their physical and chemical determination data.They were stigmasterol(â…¡),β-daucostero(â…¢),ursolic acid(â…¥),and gallic acid(â…§),these compounds were isolated from Potentilla kleiniana Wight et Arn.for the first time.