Studies On The Relationship Between The Expression Of C-Fos And MAPK Signaling Pathway In The Ovarian Carcinoma

Objective: To study the expression of c-Fos in the human ovarian carcinoma tissues and cell line HO-8910 and the role of c-Fos in the mitogen activated protein kinases(MAPKs) signal transduction in the human ovarian carcinoma.Methods: The MaxVisionTM immunohischemistry was used to observe the expression of c-Fos and phosphated extracellular-signal regulated kinase 1/2(p-ERK1/2) in the normal ovary tissues, benign ovary tissues and epithelial ovarian carcinoma. The MTT was used to observe the effect of PD98059 on the viability of human ovarian carcinoma cell line HO-8910 and the flow cytometry was utilized to observe the cell cycle. The MaxVisionTM immunocytochemistry was used to observe the expression of c-Fos in the human ovarian carcinoma cell line HO-8910. The western blot was used to observe the content of p-ERK1/2 and c-Fos in the human ovarian carcinoma cell line HO-8910.Results: Compared with the normal ovary tissues and benign ovary tissues, the contents of c-Fos and p-ERK1/2 in the epithelial ovarian carcinoma increased significantly. We also inverstigated that the expression of c-Fos and p-ERK1/2 in ovarian carcinoma were correlated with differentiation negatively (rs=-0.445, P =0.004; rs=-0.389, P <0.05 respectively), with clinical staging positively (rs=0.492, P=0.001; rs=0.521, P <0.01 respectively), with lymphatic metastasis positively (rs=0.338, P=0.033; rs=0.343, P <0.05 respectively) and had no correlation with age and histological types. The expression of c-Fos in ovarian carcinoma was correlated with p-ERK1/2 positively (rs=0.409, P<0.01). Compared with the control group, PD98059 with 150μmol/L and 200μmol/L concentration could significantly inhibit the viability of the ovarian carcinoma cell line HO-8910 (P<0.01); PD98059 could inhibit the content of p-ERK1/2 and S-phase entry of the ovarian carcinoma cell in a dose-dependent manner (P<0.01); PD98059 could also inhibit the expression of c-Fos in the cells. Conclusion: ERK1/2 might play an important role in the initiation and development of the ovarian carcinoma through c-Fos.