The Research Of The Effect That The Compound Prescription Of Yi Qi Yang Yin Huo Xue Hua Yu On The Levels Of T Cell Subpopulation Group, That Come From The Mice With Viral Myocardial Disease

Objective:To investigate the effect of the method of yi qi yang yin huo xue hua yu on the rat model of viral myocardial disease by observing the expression of T cell subset in spleen.Methods:Replicated the animal models of the viral myocardial disease with the method of injecting the CVB_3mvirus to the peritoneal cavity of the Balb/c rats for 6 times. 210 Balb/c male rats were divided into 4 groups at random:blank group,model group,low dose traditional Chinese medicine group and high dose traditional Chinese medicine group. The blank group has 30 rats.The other three group has 60 rats each.Then observe the cardiac weight index and the change of pathomorphology in the cardiac muscle tissue on the 90th day of the experiment.And flow cytometry method was used to exam the expression of CD4⁺T,CD8⁺T in spleen.Results:1.Compared with the blank group the cardiac weight index of the other three groups is higher,and compared with the model group the cardiac weight index of low dose and high dose traditional Chinese medicine group is lower.2.Compared with the blank group the changes of the collagen volume fraction in other three groups is higher.,and compared with the model group the changes of the collagen volume fraction of low dose and high dose traditional Chinese medicine group is lower.3.Compared with the blank group the expression of CD8⁺T cell in every other group is higher but the CD4⁺/CD8⁺ is lower,and compared with the model group the expression of CD8⁺ T cell in spleen in the low dose and high dose traditional Chinese medicine group is lowerbut the CD4⁺/CD8⁺ is higher.Conclusions:The prescription on the base of the method of yiqiyangyinhuoxuehuayu can lower the cardiac weight index and lower the changes of the pathomorphismand and the ultra-structure in cardiac muscle.The prescription have the effect of the immunomodulation that maybe cure viral myocardial disease.