Study On The Treatment Of Cervical Cancer Transplanted Subcutaneously In Nude Mice By Focused Ultrasound

Objective: To investigate the effect of focused ultrasound on morphology and biology of transplanted subcutaneously human cervical carcinoma in nude mice.Methods:After irradiated with different dose of focused ultrasound , the nude mice which transplanted subcutaneously with human cervical cancer were observed the growth of the subcutaneously transplanted tumor and general vital sign in each group at different time. Target areas and the rim of the targeted areas in tumor samples were examined by light microscope and electron microscopy. The immunohistochemical method was applyed to investigate the expressions of MMP-9,PCNA,,Bcl-2 and Bax .Apoptosis was detected by using TUNEL.Results: Focused ultrasound has the evident inhibitory effect on the transplanted subcutaneously human cervical carcinoma in nude mice. Denaturation, necrosis and the damage of nuclear structure were found in targeted areas of each treatment group through pathohistological observation. Tumour cell that on the rim of the targeted areas were swelling and loose arrangement. Ultrastructural investigations showed mitochondrion swelling and the formation of cytoplasm cavitation bubbles, but the change of nuclear structure was not significant. Compairing with unirradiated areas, the expressions of MMP-9 and PCNA protein were weakend, the expression of Bcl-2 was down regulated and Bax was up-regulated. Apoptosis rate of tumor cell in the rim of the targeted areas was increasing.Conclusions: Focused ultrasound could inhibit the growth of human cervical cancer transplanted subcutaneously in nude mice and induced apoptosis of tumor cell on the rim of the targeted areas significantely. The process maybe related to the mitochondria-mediated cell apoptosis that activated by focused ultrasound.